Hi Tami,
I have recently added a custom GO annotation for some specied that we use in our lab. The process is quite straightforward.
Prepare 3-column tab-delimited file of 'Entry' 'Gene names' 'Gene ontology IDs' as specified in the import dialog for custom data (ClueGO), multiple GOs per gene were separated by comma
Import the custom list via ClueGOs import feature, add the taxid for the organism (or use a dummy), add specied description in next field, and select the list file from previous step.
This worked for me on a Almalinux9 OS with Cytoscape 3.10.1 and ClueGO v2.5.10, CluePedia v1.5.10.
It did not work an the same Cytoscape/ClueGO installation on a Windows 11 OS, but I could not find out the reason for this.
Hope this helps,
Best,
Alfons