Hi Luoji,
Cytoscape recognizes a number of formats. You may structure your data as a table (tab delimited or excel), and use Import --> network from table.
Assuming substrates and products are to be represented as nodes, and EC number is the type of interaction, configure the table import dialog as follows:
Source interaction: column 2, Interaction type: column 1, Target interaction: column 2.
After the network is loaded you can set up the visual style to show the directionality of the edges.
This is the relevant manual chapter regarding network file formats and loading networks into Cytoscape:
http://www.cytoscape.org/manual/Cytoscape2_6Manual.html#Creating%20NetworksHope this helps,
Maital