Hi Parisa,
Absolutely. Cytoscape itself is agnostic about the type of data, as is clusterMaker2. Some of the other apps might make certain assumptions about the data, but for the most part you should have no problem loading in your data. Note that depending on what you are trying to do with your data, you might need a fair bit of memory. For example, to create a correlation network for that data will require the creation of a 225000X225000 matrix.
-- scooter