ongoing shortages in cytogenetics supplies

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Kath

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Oct 22, 2022, 5:10:27 AM10/22/22
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Hi everyone
our lab is having problems with many of our suppliers failing to delivery essential reagents for our lab. Things like culture flasks, pipette tips, culture media, PHA, colcemid, methanol and FISH reagents...
Currently we have shortages in locating GCT conditioned medium and colcemid. Any ideas on alternative suppliers. Or possible alternative products?
Any help would be good.

maria mouriki

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Oct 25, 2022, 4:53:03 AM10/25/22
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I don't know where your lab is located but same is here in Greece. We have to search and try new products all the time. And the more the molecular reagents multiply the cytogenetics reagents are becoming harder and harder to find. I don't know anything about the GCT medium but tell me which colcemide you are using now and who is your supplier so i may have another product/supplier to recommend.

Maria Mouriki

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EVANGELIDOU Paola

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Oct 25, 2022, 5:02:21 AM10/25/22
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Hello everyone,

 

Same situation with our lab (located in Cyprus). We have been struggling to find alternatives for quite some time now. Either because they are no longer manufactured or because they have become excruciatingly expensive. We are in the process now to try Capricorn Scientific products.

 

Regards,

 

Paola

 

Paola Evangelidou, PhD
Scientist | Cytogenetics and Genomics Department

Academic Staff | The Cyprus Institute of Neurology & Genetics
6 Iroon Avenue, 2371 Ayios Dometios, Nicosia, Cyprus | PO Box 23462, 1683, Nicosia, Cyprus
: +357 - 22 358600 (Switchboard) | :+357 - 22 392726 (Direct Line) | Fax: +357 22 392793 | pa...@cing.ac.cy | :https://www.cing.ac.cy





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From: cytogenetics-methods...@googlegroups.com <cytogenetics-methods...@googlegroups.com> On Behalf Of maria mouriki
Sent: 25 October 2022 10:12
To: cytogenetics-methods...@googlegroups.com
Subject: Re: ongoing shortages in cytogenetics supplies

 

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maria mouriki

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Oct 25, 2022, 7:27:22 AM10/25/22
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Dear Paola, i have tried the AmnioPrime medium of Capricorn on AF, CVS and POC samples and works very very well. The only thing I do extra is to add 1ml Amphotericin in 100ml of medium. You should definitely try it if you are working with such samples.

Maria Mouriki

Uloaku Chinwah

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Oct 26, 2022, 7:22:42 AM10/26/22
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Hi, 

I get colcemid from Life technologies, methanol from SLS and PHA from Qiagen Ltd. Hope this helps.

Ulo.



From: cytogenetics-methods...@googlegroups.com <cytogenetics-methods...@googlegroups.com> on behalf of Kath <kathyle...@gmail.com>
Sent: 22 October 2022 08:56
To: Cytogenetics methods and trouble-shooting Forum <cytogenetics-methods...@googlegroups.com>
Subject: ongoing shortages in cytogenetics supplies
 
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EVANGELIDOU Paola

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Oct 26, 2022, 7:22:58 AM10/26/22
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Hello Maria and thank you for your suggestion. We have actually tried AmnioPrime already and it works fine for us as well.

 

Paola

 

Paola Evangelidou, PhD
Scientist | Cytogenetics and Genomics Department

Academic Staff | The Cyprus Institute of Neurology & Genetics
6 Iroon Avenue, 2371 Ayios Dometios, Nicosia, Cyprus | PO Box 23462, 1683, Nicosia, Cyprus
: +357 - 22 358600 (Switchboard) | :+357 - 22 392726 (Direct Line) | Fax: +357 22 392793 | pa...@cing.ac.cy | :https://www.cing.ac.cy





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From: cytogenetics-methods...@googlegroups.com <cytogenetics-methods...@googlegroups.com> On Behalf Of maria mouriki
Sent: Tuesday, October 25, 2022 2:10 PM
To: cytogenetics-methods...@googlegroups.com
Subject: Re: ongoing shortages in cytogenetics supplies

 

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Dear Paola, i have tried the AmnioPrime medium of Capricorn on AF, CVS and POC samples and works very very well. The only thing I do extra is to add 1ml Amphotericin in 100ml of medium. You should definitely try it if you are working with such samples.

 

Maria Mouriki

 

Στις Τρί, 25 Οκτ 2022, 12:02 ο χρήστης 'EVANGELIDOU Paola' via Cytogenetics methods and trouble-shooting Forum <cytogenetics-methods...@googlegroups.com> έγραψε:

Hello everyone,

 

Same situation with our lab (located in Cyprus). We have been struggling to find alternatives for quite some time now. Either because they are no longer manufactured or because they have become excruciatingly expensive. We are in the process now to try Capricorn Scientific products.

 

Regards,

 

Paola

 

Paola Evangelidou, PhD
Scientist | Cytogenetics and Genomics Department
Academic Staff | The Cyprus Institute of Neurology & Genetics
6 Iroon Avenue, 2371 Ayios Dometios, Nicosia, Cyprus | PO Box 23462, 1683, Nicosia, Cyprus

(: +357 - 22 358600 (Switchboard) | (:+357 - 22 392726 (Direct Line) | Fax: +357 22 392793 | *pa...@cing.ac.cy | ü:https://www.cing.ac.cy

Kim Thompson

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Nov 4, 2022, 3:43:57 AM11/4/22
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We've been having issues with Interleukin-2 since our go to brand has been discontinued.  We are having terrible issues with all lab supplies being backordered and all our pricing quotations have not been honored.  Companies (Life tech/Thermo/Fisher) have been increasing ALL our pricing without notice multiple times over the year.

Our greatest issue now is our culture media.  We have been looking for a replacement for our MarrowMax (Life Tech) that we have been using for years.  A 100ml bottle is now $220.00 US!

Every alternative I find is produced in Europe and those companies will not ship to US due to animal products not allowed thru customs.

The cost per test for neoplastic karyotyping is insane.

I wish I had some advice and I appreciate all these replies.  Seems we are all in the same boat.

Kimberly Wolff Thompson CG(ASCP) Supervisor Clinical Specialist in Cytogenetics
C 203.240.8317 O 201.661.7280  F 201.514.1595  ktho...@PATHLINELABS.COM

     

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