How to keep cell pellets stored long-term?

[andrew]

I would like to keep blood and bone marrow cell pellets that have been
fixed for very long periods of time eg greater than 5 years and
possibly even 8 years.

The pellets must still be stored well in case we would like to make
future slides suitable for G banding.

Do you have long-term storage methods available to share? Thankyou!

[Czepulkowski Barbara (KING'S COLLEGE HOSPITAL NHS FOUNDATION TRUST)]

Dear Andrew
We store ours in little cryotubes but make sure the rubber seals are NOT on the inside of the thread. The fix eats the seals!
We keep ours for five years and have re-spread old fixed pellets successfully, and banding is also no problem.
I can find the make and name for you if you like!
Best wishes
Barbara Czepulkowski
Consultant Cytogeneticist

Sent from my iPhone

> --
> You received this message because you are subscribed to the Google Groups "Cytogenetics methods and trouble-shooting Forum" group.
> To post to this group, send an email to cytogenetics-methods...@googlegroups.com.
> To unsubscribe from this group, send email to cytogenetics-methods-and-t...@googlegroups.com.
> For more options, visit this group at http://groups.google.com/group/cytogenetics-methods-and-trouble-shooting?hl=en-GB.
>

********************************************************************************************************************

This message may contain confidential information. If you are not the intended recipient please inform the
sender that you have received the message in error before deleting it.
Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents:
to do so is strictly prohibited and may be unlawful.

Thank you for your co-operation.

NHSmail is the secure email and directory service available for all NHS staff in England and Scotland
NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients
NHSmail provides an email address for your career in the NHS and can be accessed anywhere

********************************************************************************************************************

[Majd]

Liquid Nitrogen

Sent from my iPhone

[loster]

hi andrew, we store fixed pellets in -20C.  

--
You received this message because you are subscribed to the Google Groups "Cytogenetics methods and trouble-shooting Forum" group.
To post to this group, send an email to cytogenetics-methods...@googlegroups.com.
To unsubscribe from this group, send email to cytogenetics-methods-and-t...@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/cytogenetics-methods-and-trouble-shooting?hl=en-GB.

--
Wang Hao
Cytogenetics Laboratory of Hangzhou Prenatal Diagnosis Centre,
Hangzhou First People’s Hospital.
261 Huansha Road, Shangcheng District, 
Hangzhou, Zhejiang, China.
Phone：0086-0571-87065701-10538
Fax：0086-0571-87065701-10549
Mobile： 0086-138-58068670
Email: moc...@live.cn

[Czepulkowski Barbara (KING'S COLLEGE HOSPITAL NHS FOUNDATION TRUST)]

Dear Andrew
I forgot to say we store them in a minus 20 degrees C freezer.
The micro centrifuge tubes with lids we use are from Elkay product code 000MICR150 and they are 1.5 ml capacity. Make sure they are not overfilled!
Thanks
Barbara Czepulkowski

Sent from my iPhone

On 17 May 2012, at 01:33, "andrew" <andre...@gmail.com> wrote:

> I would like to keep blood and bone marrow cell pellets that have been
> fixed for very long periods of time eg greater than 5 years and
> possibly even 8 years.
>
> The pellets must still be stored well in case we would like to make
> future slides suitable for G banding.
>
> Do you have long-term storage methods available to share? Thankyou!
>
> --
> You received this message because you are subscribed to the Google Groups "Cytogenetics methods and trouble-shooting Forum" group.
> To post to this group, send an email to cytogenetics-methods...@googlegroups.com.
> To unsubscribe from this group, send email to cytogenetics-methods-and-t...@googlegroups.com.
> For more options, visit this group at http://groups.google.com/group/cytogenetics-methods-and-trouble-shooting?hl=en-GB.
>

[chromatidx]

hi Andrew the two key factors that need to be considered is what type
of material the tube/vial consists of which will contain the stored
material and what temperature to store it:
from our observations some plastic tubes will be broken down over time
by the fixative mixture and will destroy the cells. Making slides from
this material will result in very poor spread, ghost-like metaphases
surrounded by a haze of cytoplasm. I personally recommend using
cryogenic cryovials 1mL size with an external o-ring.

Storing cells at 4C, 10C or even 20C is not enough for really
longterm storage (greater than 5 years)...I would recommend you store
the cell pellets in temperatures below - 70C.