method to remove immersion oil on uncovered slides

[oxforduni]

Hello members! Happy New Year!
Can you share a quick and easy method to effectively remove all traces
of immersion oil on uncoverslipped glass-slides. Our current method
involves blotting on lint-free paper and a quick dip in
methanol ...its not very good because lot and lots of oil residue
remains. your help is much appreciated!
bye

[Audra Birri]

We use Fisherbrand Citrisolv.
 
> Date: Tue, 25 Jan 2011 15:40:19 -0800
> Subject: method to remove immersion oil on uncovered slides
> From: gent...@gmail.com
> To: cytogenetics-methods...@googlegroups.com

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[guat lian peh]

HI We put the slide in Xylene for 5-10mins. It should remove all trace of oil. But make sure the xylene is fresh is fresh, not the same jar used to remove mountant from coverslip. Regards Guat Lian --- On Wed, 26/1/11, Audra Birri <abi...@hotmail.com> wrote:

[Šliužas Vytautas]

Hello,
we use toluene, put the slides for 20-30 minutes. You can use Coplin jar for 5 slides wach simultaneously, the same toluene could be used for 15-20 slides depending on the washed amount of oil.
Good luck!



________________________________

[EVANGELIDOU Paola]

We use Xylene

-----Original Message-----
From: cytogenetics-methods...@googlegroups.com [mailto:cytogenetics-methods...@googlegroups.com] On Behalf Of oxforduni
Sent: Wednesday, January 26, 2011 1:40 AM
To: Cytogenetics methods and trouble-shooting
Subject: method to remove immersion oil on uncovered slides

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[ESRA ATAMAN]

Hi!

You can use the xylol (xylene). (15-20m) 

--
Dr. Esra Ataman
Ege University Medical Faculty
Department of Medical Genetics
Bornova/Izmir
TURKEY

[Ala HEJAZI]

Hello..we apply a cover slip on each slide as it is stained. By that
you can wipe the coverslip with alcohol swap after analysis and
archive it directly.
Good luck!
Ala' Hejazi, MSc, CG(ASCP)

[lisagenes]

Our lab uses Safeclear II available from Fisher.

[Genetika IMZ]

Hello,

For destaining slides we use two solutions methanol with xylol and pure
metanol. We drop the solution with xylol directly onto slides with pasteur
pipette until there are no traces of oil left on the slide and then we wash
the slide in pure metanol (to wash away traces of xylol). This method is
very efficient and is used in our lab for many years.

Marija

-----Original Message-----
From: cytogenetics-methods...@googlegroups.com
[mailto:cytogenetics-methods...@googlegroups.com] On
Behalf Of Ala HEJAZI
Sent: Wednesday, January 26, 2011 5:34 PM
To: Cytogenetics methods and trouble-shooting

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