Methods for Calculating G-Band Resolution

[Ala HEJAZI]

Dear Colleagues,
I hope you are all doing well,
What is the most reliable method for calculating the G-band
resolution?
Thank you for the help.
Ala’

Ala' Hejazi, MSc, CG(ASCP)
Supervisor, Cytogenetics
Department of Pathology and Laboratory Medicine
Saad Specialist Hospital
Al-Khubar, KSA
Direct Tel: +966 3 801 2938
Mobile: +966 55 72 144 26

[melanie h]

We use the method developed in our lab (Vancouver General Hospital)
and published in AGT's journal:

Evaluation of Chromosome Banding Resolution: A Simple Guide for
Laboratory Quality Assurance, Josifek, Haessig and Pantzar. Applied
Cytogenetics17(4),1991.

It uses areas on several chromosomes to determine the overall banding
resolution - we find it quite accurate.

Cheers,

Melanie

[Fran Williams]

We use this method also and find it quite accurate.

Fran Williams, BSc., MLT
Cytogenetics Laboratory
Alberta Health Services
8-25 Medical Sciences Building
University of Alberta Hospital
Edmonton, Alberta T6G 2B7

tel: (780)407-7634 fax: (780)407-3059
email: fran.w...@albertahealthservices.ca

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Cheers,

Melanie

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[Remec]

Can you maybe post a link to this article or maybe a .pdf version of
it. I tried to find it on the internet but I couldnt find it anywhere,
not even on the AGT site.

Thank you!

ŽIga Iztok Remec

[Katie]

Hi This website gives you latest banding resolution from the ISCN2009
booklet.
www.atlasgeneticsoncology.org
I use chromosome 16 to determine resolution, if the total number of
bands in chromosome 16 is 8 or more bands it is high resolution
banding in my laboratory.

[Ala HEJAZI]

Hi Katie,
Do you count both dark and light bands or just the dark ones?
Thank you
Ala'

> > Mobile: +966 55 72 144 26- Hide quoted text -
>
> - Show quoted text -

[Winnie Lam]

You can take a look of the best practice guide of the ACT society. Winnie --- 2010年2月22日 星期一，Ala HEJAZI <a...@cytogenetechs.net> 寫道﹕ 寄件人: Ala HEJAZI <a...@cytogenetechs.net> 主題: Re: Methods for Calculating G-Band Resolution 收件人: "Cytogenetics methods and trouble-shooting" <cytogenetics-methods...@googlegroups.com> 日期: 2010年2月22日,星期一,下午2:32

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[Ala HEJAZI]

I would like to thank you all for the great help!
Warmly
Ala'

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[tom2...@gmail.com]

Our laboratory determines G-banding stage
as follows: -

400 Band stage per haploid set, e.g. two distinct bands on each of 8p
and 9p and three distinct dark bands in the middle of 5q (5q14, 5q21
and 5q23)

550 band stage per haploid set, e.g. four distinct bands on each of
18q, 3 on 11p; 7q33 and 7q35 resolve and 22q13.2 will be visible.

850 bands per haploid set are , e.g. 6q16 should split, 6q24, 6q25.2
and 6q26 appear as three distinct bands; 11p14.1 resolves from
11p14.3; 15q12 is distinct; 20p has at least two dark bands.

Sorry for the late reply to your question Ala.

> Direct Tel:              +966 3 801 2938        +966 3 801 2938
> Mobile:              +966 55 72 144 26        +966 55 72 144 26

[Ala HEJAZI]

Thank you very much tom200009 for the detailed method. I gave it 5
stars because, for me, it is practical and easy to apply.

Ala'

> > Mobile:              +966 55 72 144 26        +966 55 72 144 26- Hide quoted text -