RE: CD138 separation for multiple myeloma FISH analysis
Whitcher, Mary
We are using the EasySep human whole blood and bone marrow CD138 positive selection kit II successfully. I recommend using the DNase pre-treatment and the company’s buffer in all washes during the PCS. Once we did that, we started to get much better results.
Also, we have just started using the maximum amount of sample (2 mL) of bone marrow on all cases and the response I get from our FISH lab is encouraging.
Mary Whitcher
Senior Technologist –Cytogenomics Lab
Henry Ford Health System
Tech Town Building
440 Burroughs Suite 446
Detroit, MI
48202
From: cytogenetics-methods...@googlegroups.com <cytogenetics-methods...@googlegroups.com>
On Behalf Of Esraa
Sent: Wednesday, February 24, 2021 3:13 AM
To: Cytogenetics methods and trouble-shooting Forum <cytogenetics-methods...@googlegroups.com>
Subject: CD138 seperation for multiple myeloma FISH analysis
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Dear all,
I've used EasySep human CD138 positive selection Kit II
I end up having few CD138 cells, and when I harvested it (following AGT protocol) I couldn't see any cell under the microscope.
I'm wondering, Has anyone used the same kit and end up with many cells ?
Do you recommend EasySep human whole blood and bone marrow CD138 positive selection kit II?
What is your recommendation for the harvesting procedure to do FISH analysis after cell separation?
Thank you
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