Tris-EDTA shelf life

[Cyto.Lab.Tech]

We currently purchase Tris-EDTA buffer solution, pH 8.0 commercially from Sigma-Aldrich.  We are a clinical lab and need to have a valid expiration date for every reagent.  Since it does not have a manufacturer expiration date assigned, we currently give it a year expiration from date opened. However, find we use very little of the reagent before it expires and need to discard the rest. 

What expiration/shelf life do other labs use for this reagent?  I have seen a range of dates on-line ranging from 3months (prepared in house) to 36 months.  Any help would be appreciative. Thank you in advance!

[EVANGELIDOU Paola]

After the expiry date you set, test it again if it still works for your experiments (after validation) give it another 6 months.

 

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Paola Evangelidou
Scientist | The Cyprus Institute of Neurology & Genetics

Department of Cytogenetics and Genomics
6 International Airport Avenue, 2370 Nicosia, Cyprus  |  P.O.Box 23462, 1683 Nicosia, Cyprus
(: (+357) 22392726 |  7: (+357) 22392793 |  *: pa...@cing.ac.cy | www.cing.ac.cy

               

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[laevsky ilana]

I think, you can find the expiration date of Sigma-Aldrich's products in their web-site, for every batch. If not, try to do it from thei distributor in your country. Any way, we are freezing aliquots and thawing it before using.