Sodium Heparin vs Lithium Heparin

[Gwenevere Carter]

I've worked in Cytogenetics for over 30 years and have always heard not to collect peripheral blood samples in Lithium Heparin. A few weeks ago I received a sample that was collected in Lithium Heparin. I asked for a redraw but decided to culture the Lithium sample anyway with no special treatment of the sample. The metaphases from the Lithium sample were better than the ones from Sodium Heparin. Why do we reject the Lithium samples?

 Gwenevere Carter 

Cytogenetics Department 

Woman's Hospital 

Baton Rouge, La.

Sent from Yahoo Mail for iPhone

[Racha El-Hajj (SCHN)]

Hi everyone. Our lab for the last 20 years request and receive bone marrow and peripheral blood in only Lithium-heparin tubes.

 

 

Dr.RACHA EL-HAJJ/Hospital Scientist Cytogeneticist/cytogenetics

BSc, BAgEn,PhD,MHGSA(Cytogenetics).

T: +61 2 98453227/  e: racha....@health.nsw.gov.au

Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia
Locked Bag 4001, Westmead 2145, NSW Australia

--
You received this message because you are subscribed to the Google Groups "Cytogenetics methods and trouble-shooting Forum" group.
To unsubscribe from this group and stop receiving emails from it, send an email to cytogenetics-methods-and-t...@googlegroups.com.
To post to this group, send email to cytogenetics-methods...@googlegroups.com.
Visit this group at https://groups.google.com/group/cytogenetics-methods-and-trouble-shooting.
For more options, visit https://groups.google.com/d/optout.

This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities.

[maria mouriki]

I culture bloods taken in lithium heparin mainly but samples in sodium heparin also work well. Maybe is just a protocol that was never changed. Or maybe sodium heparin tubes are cheaper. Or someone did an experiment comparing the two types of tubes and found that samples in sodium heparin work better than the ones in lithium heparin. who knows? In conclusion both types of heparin tubes are optimal for taking blood for karyotyping.

Maria

On Mon, Jun 26, 2017 at 2:35 AM, Racha El-Hajj (SCHN) <racha....@health.nsw.gov.au> wrote:

Hi everyone. Our lab for the last 20 years request and receive bone marrow and peripheral blood in only Lithium-heparin tubes.

 

 

Dr.RACHA EL-HAJJ/Hospital Scientist Cytogeneticist/cytogenetics

BSc, BAgEn,PhD,MHGSA(Cytogenetics).

T: +61 2 98453227/  e: racha....@health.nsw.gov.au

Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia
Locked Bag 4001, Westmead 2145, NSW Australia

 

 

 

From: 'Gwenevere Carter' via Cytogenetics methods and trouble-shooting Forum [mailto:cytogenetics-methods-and-trouble-shooting@googlegroups.com]
Sent: Sunday, 25 June 2017 4:16 AM
To: cytogenetics-methods-and-trouble-shooting@googlegroups.com
Subject: Sodium Heparin vs Lithium Heparin

 

I've worked in Cytogenetics for over 30 years and have always heard not to collect peripheral blood samples in Lithium Heparin. A few weeks ago I received a sample that was collected in Lithium Heparin. I asked for a redraw but decided to culture the Lithium sample anyway with no special treatment of the sample. The metaphases from the Lithium sample were better than the ones from Sodium Heparin. Why do we reject the Lithium samples?

 Gwenevere Carter 

Cytogenetics Department 

Woman's Hospital 

Baton Rouge, La.

Sent from Yahoo Mail for iPhone

--
You received this message because you are subscribed to the Google Groups "Cytogenetics methods and trouble-shooting Forum" group.

To unsubscribe from this group and stop receiving emails from it, send an email to cytogenetics-methods-and-trouble-shooting+unsubscribe@googlegroups.com.
To post to this group, send email to cytogenetics-methods-and-trouble-shooting@googlegroups.com.

Visit this group at https://groups.google.com/group/cytogenetics-methods-and-trouble-shooting.
For more options, visit https://groups.google.com/d/optout.

This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities.

--

You received this message because you are subscribed to the Google Groups "Cytogenetics methods and trouble-shooting Forum" group.

To unsubscribe from this group and stop receiving emails from it, send an email to cytogenetics-methods-and-trouble-shooting+unsubscribe@googlegroups.com.
To post to this group, send email to cytogenetics-methods-and-trouble-shooting@googlegroups.com.

Visit this group at https://groups.google.com/group/cytogenetics-methods-and-trouble-shooting.
For more options, visit https://groups.google.com/d/optout.

--

Μουρίκη Μαρία

Κλινική Κυτταρογενετίστρια

UK Health and Care Professions Council Certified

DNA BIOLAB

2810 323034

[M. MULLEN]

This is the most baffling paradox ive run across in this field. Some labs insist on NAhep and others are sworn to lithium hep. The first lab i worked in we had very little success w/ LiHep. The one i work in now seems to show very little difference between the two. 

Seems like the insistence on Lihep is more a European thing

[Loretta Byrne]

Hi

Yes again our laboratory uses only lithium heparin samples for marrows and our failure rates are low.  I think sodium heparin is used as a preservative in lots of other areas like stem cell samples etc so mabe it was set up like that to share sample material?

T

Loretta Brown | Viapath
Deputy Head Cytogenetics
Ground Floor Cheyne Wing | King’s College Hospital | London | SE5 9RS
020 3299 7636 

020 2299 2628 (Fax)
lorett...@nhs.net

www.viapath.co.uk

GSTS and KingsPath are now Viapath.  New brand, same commitment to transforming pathology services. 

---

From: Racha El-Hajj (SCHN) <racha....@health.nsw.gov.au>
To: "cytogenetics-methods...@googlegroups.com" <cytogenetics-methods...@googlegroups.com>
Sent: Monday, 26 June 2017, 5:16
Subject: RE: Sodium Heparin vs Lithium Heparin

[Anurag Gupta]

Hi guys,

 

An interesting discussion. Wanted to understand what is the average time between sample collection in lithium heparin and culture set ups?

 

Thanks and regards

 

Dr. Anurag Gupta.

MD.Path. (TMNC, Mumbai), FCCG (Clinical and Cancer Cytogenetics, Tata Medical Center Kolkata), MBBS (AFMC Pune),
PGDHHM, PGDMLS.

Consultant Cytogenetics and  Pathology

Dept. of Cytogenetics

AMPATH, 

American Oncology Institute and Citizens Hospital 

Serilingampally, Hyderabad

 

 

Sent from my Windows 10 phone

 

From: 'Loretta Byrne' via Cytogenetics methods and trouble-shooting Forum
Sent: 27 June 2017 15:42
To: cytogenetics-methods...@googlegroups.com
Subject: Re: Sodium Heparin vs Lithium Heparin

 

Hi

 

Yes again our laboratory uses only lithium heparin samples for marrows and our failure rates are low.  I think sodium heparin is used as a preservative in lots of other areas like stem cell samples etc so mabe it was set up like that to share sample material?

 

T

Loretta Brown | Viapath
Deputy Head Cytogenetics
Ground Floor Cheyne Wing | King’s College Hospital | London | SE5 9RS
020 3299 7636 

020 2299 2628 (Fax)
lorett...@nhs.net

www.viapath.co.uk

GSTS and KingsPath are now Viapath.  New brand, same commitment to transforming pathology services. 

 

[Suguru Revathi]

Hi everyone, I am also working in genetics.And I have cultured some of the genetic samples in Lithium only

 got better results than Sodium Hep.

Thank you All

--

You received this message because you are subscribed to the Google Groups "Cytogenetics methods and trouble-shooting Forum" group.

To unsubscribe from this group and stop receiving emails from it, send an email to cytogenetics-methods-and-trouble-shooting+unsubscribe@googlegroups.com.
To post to this group, send email to cytogenetics-methods-and-trouble-shooting@googlegroups.com.

[Whitcher, Mary]

My experience of culturing bloods and bone marrows is that Lithium Heparin lowers the mitotic index.  We have a special procedure for when samples come in Lithium instead of Sodium of washing and doubling up on the mitogens to compensate.

 

Mary Whitcher

Sr. Technologist- Cytogenetics Lab

Henry Ford Health System

Detroit, MI

 

 

 

From: 'Gwenevere Carter' via Cytogenetics methods and trouble-shooting Forum [mailto:cytogenetics-methods...@googlegroups.com]
Sent: Sunday, 25 June 2017 4:16 AM
To: cytogenetics-methods...@googlegroups.com
Subject: Sodium Heparin vs Lithium Heparin

 

I've worked in Cytogenetics for over 30 years and have always heard not to collect peripheral blood samples in Lithium Heparin. A few weeks ago I received a sample that was collected in Lithium Heparin. I asked for a redraw but decided to culture the Lithium sample anyway with no special treatment of the sample. The metaphases from the Lithium sample were better than the ones from Sodium Heparin. Why do we reject the Lithium samples?

 Gwenevere Carter 

Cytogenetics Department 

Woman's Hospital 

Baton Rouge, La.

Sent from Yahoo Mail for iPhone

--
You received this message because you are subscribed to the Google Groups "Cytogenetics methods and trouble-shooting Forum" group.
To unsubscribe from this group and stop receiving emails from it, send an email to cytogenetics-methods-and-t...@googlegroups.com.
To post to this group, send email to cytogenetics-methods...@googlegroups.com.
Visit this group at https://groups.google.com/group/cytogenetics-methods-and-trouble-shooting.
For more options, visit https://groups.google.com/d/optout.

This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities.

--
You received this message because you are subscribed to the Google Groups "Cytogenetics methods and trouble-shooting Forum" group.
To unsubscribe from this group and stop receiving emails from it, send an email to cytogenetics-methods-and-t...@googlegroups.com.
To post to this group, send email to cytogenetics-methods...@googlegroups.com.
Visit this group at https://groups.google.com/group/cytogenetics-methods-and-trouble-shooting.
For more options, visit https://groups.google.com/d/optout.

---

CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies.

Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy & Security page on www.henryford.com for more detailed information as well as information concerning MyChart, our new patient portal. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us.

[sanjaci]

Hi, everyone,

I heard that story (Na vs. Li) more then 12 years ago, but recently I've found one of possible explanation for this (maybe in this forum, in other topics). Someone wrote that Li-heparine vacuettes had much more concentration of Li, and cells did not grow (our lab had that problem). As I said it was long ago, now it is different situation (I think we receive both, but I'll check it), the only important thing is that blood for cytogenetics is with heparine.

Best regards,

Sanja

[Iqbal, Anwar]

Lithium is a chelating agent and can induce clotting which will interfere with cell cultures.

Hope this is helpful.

Anwar

--

[ramakrishnan sasi]

 I am not sure Li is considered as an intercalator or chelating agent. Usually, chelators are bifunctional with two binding sites to trap a molecule like EDTA or EGTA( my chemistry 101).

Li is monovalent ( LI+) . Lithium resembles magnesium because of their similar size (similar ionic density). Perhaps it may bind more strongly to other –negatively charged molecules like Mg++.  But  I am afraid it may not behave like a bifunctional chelating agent or form a cage-like structure to trap molecules.

 

Ramakrishnan Sasi,

West Virginia University

 

---

From: "Iqbal, Anwar" <Anwar...@URMC.Rochester.edu>

To: Cytogenetics methods and trouble-shooting Forum <cytogenetics-methods...@googlegroups.com>

Sent: Thursday, June 29, 2017 6:15 AM
Subject: RE: Sodium Heparin vs Lithium Heparin