Cytogenetics methods and trouble-shooting Forum

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CYTOGENETICS METHODS & TROUBLE-SHOOTING discussion group is a friendly and practical group for all discussions about:

human blood/bone marrow/solid tumour/prenatal/skins/POC methodologies.

Its the largest cytogenetics discussion group on the Web with well over 1400+ members. Becoming a member of the group is totally FREE.

Additionally, this is a forum to discuss cytogenetic problems such as harvesting issues, chromosme banding, karyotypes, microscopy and cell culture issues as they apply

to the field of cytogenetics.

This is also a forum for all discussions about plant and animal cytogenetics, as well as any chromosome research issues.

Molecular Cytogenetics topics are also welcomed by the group.

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Feb 22

Genial Genetics and EuroClone

Hello, can anyone please tell me where I can buy Genial Genetics and EuroClone products in the USA? I

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Genial Genetics and EuroClone

Hello, can anyone please tell me where I can buy Genial Genetics and EuroClone products in the USA? I

Feb 22

5/16/23

Improved NUP98 break-apart probes

You email me at akbyn...@yahoo.com. Thanks, On Monday, May 15, 2023 at 3:10:59 AM UTC-7 Jay wrote:

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Improved NUP98 break-apart probes

You email me at akbyn...@yahoo.com. Thanks, On Monday, May 15, 2023 at 3:10:59 AM UTC-7 Jay wrote:

5/16/23

5/16/23

Hello, I'm trying to set up an RNA fish assay to see the whole cell, I mean not just the nucleus.

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Hello, I'm trying to set up an RNA fish assay to see the whole cell, I mean not just the nucleus.

5/16/23

K H Ramesh, … Racha El-Hajj (SCHN)6

4/23/23

Plasma Cell Enriched - FISH

Dear Mark: Thank you. Can you send me a phone # where I can reach you. Thanks. Ramesh From:

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Plasma Cell Enriched - FISH

Dear Mark: Thank you. Can you send me a phone # where I can reach you. Thanks. Ramesh From:

4/23/23

4/15/23

Alternation to DIG labeled probe amplification

Hellou everyone, I have a quenstion regarding Dig labelled probe amplification. We used to use DIG

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Alternation to DIG labeled probe amplification

Hellou everyone, I have a quenstion regarding Dig labelled probe amplification. We used to use DIG

4/15/23

perla natalia Vargas, … DANIEL ANDRES FABIAN VILLAGOMEZ ZAVALA7

4/9/23

Harvesting long chromes

Thank you so much! Will le y your know how it goes! On Sat, Apr 8, 2023 at 08:00 DANIEL ANDRES FABIAN

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Harvesting long chromes

Thank you so much! Will le y your know how it goes! On Sat, Apr 8, 2023 at 08:00 DANIEL ANDRES FABIAN

4/9/23

Miliausha Tagirova, Khalil Rashidi2

4/6/23

Invitation to join free Russian Cytogenomics Conference with international participation (June 2023, Saint-Pterbourg)

My name is Syed Khalil Rashidi, and I am a Ph.D. student in medical biotechnology from the Semnan

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Invitation to join free Russian Cytogenomics Conference with international participation (June 2023, Saint-Pterbourg)

My name is Syed Khalil Rashidi, and I am a Ph.D. student in medical biotechnology from the Semnan

4/6/23

kathren, … Jee H KYHM6

3/19/23

premade hypotonic solutions?

Please go to the link below https://www.thermofisher.com/order/catalog/product/10575090?SID=srch-srp-

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premade hypotonic solutions?

Please go to the link below https://www.thermofisher.com/order/catalog/product/10575090?SID=srch-srp-

3/19/23

ritu rao, … maria mouriki6

2/15/23

This is most likely double stalks and satellites. You could perform C and NOR but i don't believe

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This is most likely double stalks and satellites. You could perform C and NOR but i don't believe

2/15/23

Kath, … Kim Thompson7

11/4/22

ongoing shortages in cytogenetics supplies

We've been having issues with Interleukin-2 since our go to brand has been discontinued. We are

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ongoing shortages in cytogenetics supplies

We've been having issues with Interleukin-2 since our go to brand has been discontinued. We are

11/4/22

10/22/22

karyotype of post-allo-HSCT

Dear all, A case acute leukemia, Female; which was diagnosed as AML with t(8;21) and RUNX1::RUNX1T1

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karyotype of post-allo-HSCT

Dear all, A case acute leukemia, Female; which was diagnosed as AML with t(8;21) and RUNX1::RUNX1T1

10/22/22

sunil kumar polipalli

10/21/22

Regarding Triplex DNA Fiber FISH technology

Dear All, Can anyone share the details pertaining to the methodology of Triplex DNA Fiber FISH

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Regarding Triplex DNA Fiber FISH technology

Dear All, Can anyone share the details pertaining to the methodology of Triplex DNA Fiber FISH

10/21/22

Chin Lee Phan, kathren3

10/20/22

How to perform measurement uncertainty (cut-off value) for FISH probe

Hi , Thank you very much On Wed, 19 Oct 2022 at 20:06, kathren <kand...@gmail.com> wrote: Hi

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How to perform measurement uncertainty (cut-off value) for FISH probe

Hi , Thank you very much On Wed, 19 Oct 2022 at 20:06, kathren <kand...@gmail.com> wrote: Hi

10/20/22

8/29/22

CME & Hands on FISH workshop on 14th and 15 september 2022

Dear All, Kindly Find attached the brochure for CME and Workshop for Cancer Cytogenetics, conducted

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CME & Hands on FISH workshop on 14th and 15 september 2022

Dear All, Kindly Find attached the brochure for CME and Workshop for Cancer Cytogenetics, conducted

8/29/22

8/28/22

New private Cytogenetics lab in Sydney

Hi i am trying to locate the advertisement for a new private cytogenetics lab startup in Sydney.

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New private Cytogenetics lab in Sydney

Hi i am trying to locate the advertisement for a new private cytogenetics lab startup in Sydney.

8/28/22

3/1/22

how to improve growth in blood cultures?

Members can you help me locate this article: Anaemia in older people Angela M. Sanford Book Editor(s)

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how to improve growth in blood cultures?

Members can you help me locate this article: Anaemia in older people Angela M. Sanford Book Editor(s)

3/1/22

2/1/22

cytogenetics posting @ Yale

Good morning! Cytogenetics lab @ Yale has an entry level position open for a cytogenetics tech. The

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cytogenetics posting @ Yale

Good morning! Cytogenetics lab @ Yale has an entry level position open for a cytogenetics tech. The

2/1/22

K H Ramesh, majd2

1/20/22

FISH Automation

Biodot technologies On Thu, Jan 20, 2022 at 3:25 AM KH Ramesh <KRA...@montefiore.org> wrote:

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FISH Automation

Biodot technologies On Thu, Jan 20, 2022 at 3:25 AM KH Ramesh <KRA...@montefiore.org> wrote:

1/20/22

Ковалева Наталия

1/14/22

Dear colleagues, I need very bad a paper unavailable here. May somebody help me to get this one? BR,

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Dear colleagues, I need very bad a paper unavailable here. May somebody help me to get this one? BR,

1/14/22

Tony, … Iqbal, Anwar3

1/12/22

COVID Safety advise required - pregnant staff working in Cytogenetics

Here is a link: Q&A with Dr. Eva Pressman About Pregnancy, the Vaccine and More - URMC Operations

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COVID Safety advise required - pregnant staff working in Cytogenetics

Here is a link: Q&A with Dr. Eva Pressman About Pregnancy, the Vaccine and More - URMC Operations

1/12/22

Mizanur Rahman, … Ковалева Наталия9

1/8/22

Regarding Y chromosome

Referral reason: Infertility and semen shows azoospermia. Regards Brig Gen (Dr) Md Mizanur Rahman

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Regarding Y chromosome

Referral reason: Infertility and semen shows azoospermia. Regards Brig Gen (Dr) Md Mizanur Rahman

1/8/22

ritu rao, … Güven Toksoy3

12/2/21

Hi, In my opinion, if cryptic changes are ignored, this can be considered at(1;17)(p34.?;p13?)

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Hi, In my opinion, if cryptic changes are ignored, this can be considered at(1;17)(p34.?;p13?)

12/2/21

8/15/21

resurrecting verry poor sub-optimal bone marrow samples

Is the BMGS the same supplement sold by Genial the same as BMGS sold by United Biosciences? On Friday

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resurrecting verry poor sub-optimal bone marrow samples

Is the BMGS the same supplement sold by Genial the same as BMGS sold by United Biosciences? On Friday

8/15/21

ritu rao, … Paul Oei4

7/13/21

Looks like r(21). Dynamic instability seen here would also fit with ring.. This e-mail may be

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Looks like r(21). Dynamic instability seen here would also fit with ring.. This e-mail may be

7/13/21

Citogenetica Salvador

7/6/21

Dear all. A 65 year old patient, with acute myeloid leukemia, present the next karyotype (our

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Dear all. A 65 year old patient, with acute myeloid leukemia, present the next karyotype (our

7/6/21

5/31/21

correct referencing a forum

Hi guys how do i reference a specific conversation on this forum when i want to use it in a science

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correct referencing a forum

Hi guys how do i reference a specific conversation on this forum when i want to use it in a science

5/31/21

stuart, … simon3

4/19/21

how do i rescue a poor quality or sub-optimal oncology specimens?

Old blood specimens may contain non-visible "miro-clots" so i suppose that is why treating

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how do i rescue a poor quality or sub-optimal oncology specimens?

Old blood specimens may contain non-visible "miro-clots" so i suppose that is why treating

4/19/21

manisha desai, … Dominiqu...@radboudumc.nl5

3/26/21

Inversion in chromosome 11

Dear Dr. Dominique Thank you so much for your immediate response and valuable opinion. This surely

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Inversion in chromosome 11

Dear Dr. Dominique Thank you so much for your immediate response and valuable opinion. This surely

3/26/21

Suguru Revathi, … maria mouriki5

2/27/21

Fwd: Abnormal X Chromosome

Have you run any additional tests on this patient (FISH with X subtelomeres probes or X chromosome

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Fwd: Abnormal X Chromosome

Have you run any additional tests on this patient (FISH with X subtelomeres probes or X chromosome

2/27/21

2/25/21

RE: CD138 separation for multiple myeloma FISH analysis

We are using the EasySep human whole blood and bone marrow CD138 positive selection kit II

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RE: CD138 separation for multiple myeloma FISH analysis

We are using the EasySep human whole blood and bone marrow CD138 positive selection kit II

2/25/21

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