Bioprocess Engineering By Shuler And Kargi
Yamila Comejo
The authors review relevant fundamentals of biochemistry, microbiology, and molecular biology, including enzymes, cell functions and growth, major metabolic pathways, alteration of cellular information, and other key topics. They then introduce evolving biological tools for manipulating cell biology more effectively and to reduce costs of bioprocesses.
This edition presents major advances in the production of biologicals; highly productive techniques for making heterologous proteins; new commercial applications for both animal and plant cell cultures; key improvements in recombinant DNA microbe engineering; techniques for more consistent authentic post-translational processing of proteins; and other advanced topics. It includes new, improved, or expanded coverage of
This concise yet comprehensive text introduces the essential concepts of bioprocessing internal structure and functions of different types of microorganisms, major metabolic pathways, enzymes, microbial genetics, kinetics and stoichiometry of growth and product information to traditional chemical engineers and those in related disciplines. It explores the engineering principles necessary for bioprocess synthesis and design, and illustrates the application of these principles to modern biotechnology for production of pharmaceuticals and biologics, solution of environmental problems, production of commodities, and medical applications.
Bioprocess Engineering, Third Edition, is an extensive update of the world's leading introductory textbook on biochemical and bioprocess engineering and reflects key advances in productivity, innovation, and safety.
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The origins of biomolecular engineering, however, date back much earlier to the first half of the twentieth century, with the first use of process engineering principles to extract products from biological systems. A prominent early example is the large-scale production of penicillin during the 1940s amidst the Second World War2,3. The finicky nature of this antibiotic posed several major design challenges for bioprocess engineers and researchers alike. These included identifying a microbial strain that maximized product yield, designing large-scale fermenters with optimized reaction conditions, recovering desired products from the fermentation broth and preventing microbial contamination throughout the entire process2. Applying such principles to biological systems remains a cornerstone of contemporary biomolecular engineering; some more recent examples include the large-scale production of mRNA vaccines for COVID-19 (ref. 3) and the design of a microbial gas-fermentation process for commodity chemical production4.
In this issue of Nature Chemical Engineering, we feature another example of modern biomolecular engineering, here combining synthetic pathway design, metabolic engineering and bioprocess engineering. Featured on the cover and in an Article by Choi and co-workers, benzyl acetate, a small aromatic ester often used in flavors and fragrances, is produced from d-glucose in metabolically engineered Escherichia coli. This team of researchers designed two biosynthetic pathways consisting of upstream and downstream modules that convert metabolites of E. coli into benzyl acetate, as it does not naturally produce this compound. A delayed co-culture strategy was then developed to optimize production of the upstream and downstream strains, whose performance was assessed in a two-phase extractive fed-batch fermentation. As noted in a News & Views by Sokolova and Haslinger, this strategy, involving delayed inoculation of one strain, led to major titer and selectivity enhancements, but future work is needed to show that this strategy can be implemented at an industrial scale.
Indeed, today, this sub-field covers a diverse array of topics, including tissue engineering, systems biology, protein engineering, regenerative medicine and metabolic engineering, to name a few. Our pre-launch collection features 15 recent Articles on biomolecular engineering from the Nature Portfolio to give a clearer view of intended topical coverage.
Dr. Michael L. Shuler is Samuel B. Eckert Professor of Engineering at Cornell University. He directed the School of Chemical Engineering (1998-2002) and was founding James and Marsha McCormick Chair for Biomedical Engineering (2004-2014). He also directs the Center on the Microenvironment and Metastasis (CMM), funded by the National Cancer Institute as a Physical Sciences - Oncology Center. He has received numerous teaching, advising, and research related awards, and has been elected to the National Academy of Engineering and the American Academy of Arts and Sciences.
Fikret Kargi is Professor in the Department of Environmental Engineering at Dokuz Eylul University. His interests include bioprocess engineering, environmental biotechnology, wastewater treatment, biotechnology-bioengineering, and waste bioprocessing. He holds a Ph.D. in Chemical/Biochemical Engineering from Cornell.
Bioprocess Engineering, Second Edition is a comprehensive update of the world's leading introductory textbook on biochemical and bioprocess engineering. Drs. Michael L. Shuler and Fikret Kargi review the relevant fundamentals of biochemistry, microbiology, and molecular biology, introducing key principles that enable bioprocess engineers to achieve consistent control over biological activity. This edition reflects powerful advances that are transforming the field, ranging from genetic sequencing to new techniques for producing proteins from recombinant DNA. It introduces techniques with broad application to the production of pharmaceuticals, biologics, and commodities; to medical applications such as tissue engineering and gene therapy; and for solving critical environmental problems. This new edition includes:
Bioprocess Engineering, Second Edition makes extensive use of illustrations, examples, and problems, and contains extensive references for further reading as well as a detailed appendix describing traditional bioprocesses.
On completion of this module students should be able:
1.To describe the importance of bioprocess engineering in pharmaceutical, biopharmaceutical production, food processing and environmental processes.
2.To describe the importance of sterilisation, GMP and regulatory bodies in the production of products for human use.
3.To describe the types of microorganisms used in industrial bioprocesses and discuss their growth requirements.
4.To demonstrate an understanding of the techniques for measurement and monitoring of parameters relevant to the quantitative analysis of bio reaction processes.
5. To demonstrate an understanding of the role of bioprocess engineering in sustainability and bio circular economy.
6.To apply stoichiometric principles for macroscopic analysis of cell growth and production.
7.To write appropriate equations for conservation of mass for bioprocesses with and without reaction for both steady-state and unsteady state.
8.To demonstrate a quantitative understanding of basic principles of heat transfer in bioprocesses including heat sterilisation techniques.
9.To outline the steps involved in a typical industrial bioprocess and describe both upstream and downstream unit operations.
10.To describe the industrial production of penicillin and alcohol.
11.To demonstrate the ability to work together as a team and present results in the form of a poster and oral presentation.