Demultiplexing on terra - error code 7

[Damasio, Marcos]

Good afternoon,

I have been trying to demultiplex my data on Terra Bio for the RUN_1168 folder 210117 and when I run the analysis, I get the following error:

Task bcl2fastq.run_bcl2fastq:NA:1 failed. The job was stopped before the command finished. PAPI error code 7. Execution failed: generic::permission_denied: pulling image: docker pull: running ["docker" "pull" "gcr.io/broad-cumulus/bcl2fastq:2.20.0.422"]: exit status 1 (standard error: "Error response from daemon: pull access denied for gcr.io/broad-cumulus/bcl2fastq, repository does not exist or may require 'docker login': denied: Permission denied for \"2.20.0.422\" from request \"/v2/broad-cumulus/bcl2fastq/manifests/2.20.0.422\".\n”)

What can be the reason for the error? Any help is extremely appreciated.

Thank you so much

Marcos

Marcos Damasio, PhD
Postdoctoral Research Fellow
Gastrointestinal Unit
Massachusetts General Hospital
Harvard Medical School
Warren 1019A
55 Fruit Street
Boston MA 02114

mdam...@mgh.harvard.edu

[Joshua Gould]

Hi, please see https://cumulus.readthedocs.io/en/stable/bcl2fastq.html.
Alternatively you can run cellranger mkfastq on your server and use
cumulus only for the count part.

Best,

Josh

> --
> You received this message because you are subscribed to the Google Groups "Cumulus Support" group.
> To unsubscribe from this group and stop receiving emails from it, send an email to cumulus-suppo...@googlegroups.com.
> To view this discussion on the web visit https://groups.google.com/d/msgid/cumulus-support/44D0EC3A-63F3-4DDD-96E0-43E471702ED5%40mgh.harvard.edu.