mutation.random.0.bed failed: 6400

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Cai Qi

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Dec 9, 2020, 3:47:10 PM12/9/20
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Hi All:

I am using CTK to analyze a dataset at the step of getting CIMS, I cannot get the "XXX.CIMS.txt"file but some files generated in the middle I guess.

I looked into the process, it looks it fails at:

writing profile to ./CIMS.pl_1607545853_0.160529621950449/mutation.tagcount.bed ...

reading frequency of mutations and tag number for each mutation site

0 ...

0 ...

find cummulative distributions ...

start simulations, 96745 mutations in total  ...

Iteration 0 ...

generating random CIMS sites ...

perl /home/caiqi/ctk/simulateCIMS.pl -n 96745 -w 1  -p ./CIMS.pl_1607545853_0.160529621950449/mutation.pos.txt  -srand 1.17082803610629 HePG2.HNRNPK.pool.R2.tag.uniq.rgb.bed ./CIMS.pl_1607545853_0.160529621950449/mutation.random.0.bed

position < 0?

 at /home/caiqi/ctk/simulateCIMS.pl line 66, <$fin> line 1.

CMD=perl /home/caiqi/ctk/simulateCIMS.pl -n 96745 -w 1  -p ./CIMS.pl_1607545853_0.160529621950449/mutation.pos.txt  -srand 1.17082803610629 HePG2.HNRNPK.pool.R2.tag.uniq.rgb.bed ./CIMS.pl_1607545853_0.160529621950449/mutation.random.0.bed failed: 6400

 at /home/caiqi/ctk/CIMS.pl line 229.


What should I do to fix the problem?


Thanks,

Cai

Si Mi

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Jan 31, 2023, 11:37:00 AM1/31/23
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Hello Cai,
I met the same problem. Did you solve it at last?

Thanks,
Si

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