Why eclip-seq only analyze the read2

Cai Qi

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Nov 26, 2020, 1:03:10 PM11/26/20

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Hi,

I have just went through most part of the eCLIP-analysis using CTK protocol, and I noticed that in the tutorial, only Read2 were used for analysis. I have seen this in other turorial as well. I am wondering is there any specific reason that Read1 is not used?

Thanks a lot,

Cai

Chaolin Zhang

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Nov 26, 2020, 2:00:55 PM11/26/20

to Cai Qi, CTK User Group

Reads 2 is the sense strand, capturing the 5’end of the CLIP tag. The start position has the information of the exact crosslink sites.

Chaolin

On Nov 26, 2020, at 1:03 PM, Cai Qi <qica...@gmail.com> wrote:

Hi,

I have just went through most part of the eCLIP-analysis using CTK protocol, and I noticed that in the tutorial, only Read2 were used for analysis. I have seen this in other turorial as well. I am wondering is there any specific reason that Read1 is not used?

Thanks a lot,

Cai

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Cai Qi

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Nov 26, 2020, 2:15:32 PM11/26/20

to Chaolin Zhang, CTK User Group

Thanks, Dr. Zhang!

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Cai Qi
Neuroscience Research Institute, Peking University
Xueyuan road, Beijing, China
100191

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