problem encountered trying cryo-EM in the cloud_ .aws_relion folder missing?

[ldel...@gmail.com]

Hi,
I am trying your new Cryo-EM tools to submit jobs from relion GUI and although I think I have followed carefully setup instructions I am getting the following error when trying to execute unblur:

2018/01/31 13:21:34 Attempt 1/3 failed with 1 errors and: InvalidBucketName: The specified bucket is not valid.
        status code: 400, request id: 3A065269EB4BF4E4
2018/01/31 13:21:34 Attempt 2/3 failed with 1 errors and: InvalidBucketName: The specified bucket is not valid.
        status code: 400, request id: E1700B08AA4BB574
2018/01/31 13:21:34 Attempt 3/3 failed with 1 errors and: InvalidBucketName: The specified bucket is not valid.
        status code: 400, request id: F105C2373798FD81
2018/01/31 13:21:34 Failed to copy: InvalidBucketName: The specified bucket is not valid.
        status code: 400, request id: F105C2373798FD81
Traceback (most recent call last):
  File "relion/qsub_aws", line 4567, in <module>
  File "relion/qsub_aws", line 3369, in relion_run_motioncorr
ZeroDivisionError: float division by zero
[24999] Failed to execute script qsub_aws

Looking in the code I can see everywhere that folder .aws_relion should exists but I cannot find it anywhere nor I found instructions on where and how to create it.

Am I missing something?

thanks
Laura

[Michael Cianfrocco]

Hi Laura, 

Did you add 'S3FullAccess' permissions using AWS IAM ? Just to make sure that you can write to S3.

(From this page: http://cryoem-tools.cloud/getting-started-on-aws/#Set_up_user_accounts)

Regarding the .aws_relion file, that will be created eventually but is not required in order to launch the code.

Mike 

[ldel...@gmail.com]

Hi Mike,
in fact I did not have that permission on my IAM user. I have added it, remake Access key and tried again but I am still getting the same error:

2018/02/16 14:23:37 Attempt 1/3 failed with 1 errors and: InvalidBucketName: The specified bucket is not valid.
        status code: 400, request id: 2D3E47F203D5E49B
2018/02/16 14:23:38 Attempt 2/3 failed with 1 errors and: InvalidBucketName: The specified bucket is not valid.
        status code: 400, request id: 9C822CDD72EA22EF
2018/02/16 14:23:38 Attempt 3/3 failed with 1 errors and: InvalidBucketName: The specified bucket is not valid.
        status code: 400, request id: 3744114A2D793486
2018/02/16 14:23:38 Failed to copy: InvalidBucketName: The specified bucket is not valid.
        status code: 400, request id: 3744114A2D793486

Traceback (most recent call last):
  File "relion/qsub_aws", line 4567, in <module>
  File "relion/qsub_aws", line 3369, in relion_run_motioncorr
ZeroDivisionError: float division by zero

[7826] Failed to execute script qsub_aws

Do I need to start by importing the movies so they get to the S3 bucket? I got the impression that movies are uploaded into S3 when trying to align them (unblur/motioncor2) but maybe I need to import them before using that queue_aws.

thanks
Laura