Hi group,
I've started using crux 1.37 but ran into problems as all xcorrs in the sqt-files after my sequest-searches turn out to be zero. Here's an example:
S 1374 1374 3 0.0 server 1449.7317 0.00 0.00000000 3970
M 1 1 1449.7212 nan 0 79.219582 8 48 Q.AIDHAVQLSRDEP.S U
L fid|6014821|locus|ECBD_1912|
Apart from that, the search worked fine. I used the following parameters:
precursor-window=15
precursor-window-type=ppm
isotopic-mass=mono
fragment-mass=mono
mod=15.994915:M:4
enzyme=no-enzyme
num-decoys-per-target=0
mz-bin-width=0.36
mz-bin-offset=0.11
I saw this for a few different ms2-files, do you have an idea of why this happens? Tell me if you'd need more information.
Thank you,
Viktor