SKEW RATIO GREATER THAN 10 with GeCKO v2

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Ignacio Cardona Benavides

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Jul 7, 2023, 3:44:19 AM7/7/23
to Genome Engineering using CRISPR/Cas Systems
Hello,

In our laboratory we have done a positive screening with a drug. The sequencing data with the complete library (A+B) of GeCKo v2 in the case of treatment with the drug (FAR) in the 3 replicates have been:
Captura de pantalla 2023-07-07 094204.png


We are not sure whether further sequencing analysis would be appropriate. The quality parameters given by the sequencer were correct.

Thank you very much! 
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