Does doubling time matters for drug resistance CRISPR study

[Suman Mukhopadhyay]

Dear all,

I have a quick question: am using two different cell lines (H2122 and H358) for my CRISPR drug resistance study. H2122 grows faster (doubling time 24 hrs) while H358 takes little extra time (doubling time 38 hrs).

So after puro selection is done for 1 week, while starting drug treatment do I need to cae about doubling time, to keep both of lines in same doubling time under drug treatment? or it does not matter since each one have their own control sets. For example, based on my drug treatment preliminary experiment, I am planning to treat H2122 for 10 days and H358 for 14 days because those days came out as good for drug concentrations.  So does it matter as both of the lines will have different doubling time under drug treatment.

At the end of the day, I will compare both lines data. 

Need some suggestion on this.

Thanks,

Suman