sgRNA amplification & NGS

[didem tecimel]

Hi everyone, 

I am using the GeCKO v2 library and following the protocol from Joung et al. Nature 2017. In this protocol, primer sequences for amplifying the sgRNA library and NGS. However, the given Rev primers add the barcodes for Illumina. In our laboratory, we will use MGI-DNBSeq400 ( BGI-Research, Shenzhen) for sequencing. 

I would appreciate any experience and guidance you can give me regarding the library prep.

Best wishes,

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Didem TECIMEL, M.Sc.

PhD student

Yeditepe University School of Medicine

Department of Medical Genetics

Istanbul / TURKEY