can I transfect the lentiCRISPR v2 with VSV-G and pCMV-dR8.9 packaging plasmid in 293 K cells?

[hongz...@gmail.com]

Hi to anyone has the experience using LentiCRISPR v2,

I have questions about the lentiCRISPR v2, 

1) can I transfect the lentiCRISPR v2 with VSV-G and pCMV-dR8.9 packaging plasmid in 293 K cells?

2) do I need the empty vector for negative control? 

thanks!!

Hongzhi

[CB]

293 k? Do you mean 293 or 293T?

[hongz...@gmail.com]

293K

[CB]

What is is 293K. I have never heard of this 293 version.

[hongz...@gmail.com]

sorry, it is 293T cells.

[CB]

293T is primarily used for lenti packaging. I've used lentiCRISPR with psPAX2 and pMD2.G to transfect 293T and make virus.

[Julia Joung]

Hi Hongzhi,

We have put together a screening protocol that includes lentiviral production that you might find helpful here. I have also recently found that Lipofectamine 3000 is a better alternative to lipofectamine 2000, and am attaching the updated protocol using lipofectamine 3000.

Best,

Julia

On Thu, Aug 17, 2017 at 7:48 AM, CB <carter...@gmail.com> wrote:

293T is primarily used for lenti packaging. I've used lentiCRISPR with psPAX2 and pMD2.G to transfect 293T and make virus.

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