Hello, fellow researchers,
I'm working on a project cloning 11 guide RNAs into a Pxr003 plasmid using BbsI enzyme for cutting. How can I best approach this to ensure successful ligation of all guides? Are there any specific protocols or considerations I should be aware of? Do you have any tips on optimizing gRNA selection and ensuring efficient digestion and ligation with BbsI?
Appreciate your expertise!
![image.png](https://groups.google.com/group/crispr/attach/c44b4cfd9bed/image.png?part=0.1&view=1)
Salima Abu Rabe'a, M.Sc. student
Prof. Gil Ast laboratory
Departure of Human Molecular Genetics & Biochemistry
Sackler Faculty of Medicine
Tel Aviv University, Israel