Can dCas9-VPR, instead of dCAS-VP64, be used with Calabrese P65-HSF gRNA library???
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h x
Jul 14, 2021, 8:20:21 PM7/14/21
to Genome Engineering using CRISPR/Cas Systems
http://www.addgene.org/pooled-library/broadgpp-human-crispra-calabrese-p65hsf/
On this website of the gRNA library, it is mentioned “A dCas9 activation plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with lenti dCAS-VP64_Blast (Addgene #61425).” Then I wonder if I can use dCas9-VPR instead of dCas9-VP64.
Does anyone have relevant experience to share?
Thank you very much!
Aurelien Serandour
Oct 7, 2021, 5:34:26 AM10/7/21
to Genome Engineering using CRISPR/Cas Systems
I am wondering the same, did you have any answer?
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