Online course: Introduction to CRISPR for Ecology and Evolution Studies

[Carlo Pecoraro]

Dear all,

 

registrations are open for the 2nd edition of the online course "Introduction to CRISPR for Ecology and Evolution Studies".

 

Dates: 14-18 October

 

Course website: https://www.physalia-courses.org/courses-workshops/course53/

 

The course is intended for researchers interested in implementing the CRISPR-Cas9 tool in their own projects. There will be theoretical lectures and hands-on practical exercises using web-based tools, license-free software, and UNIX-based command line programs. Experience using command-line based programs will be advantageous, but not required. An introduction to UNIX commands will be provided.

 

At the end of this course, attendees will be in the position to:

 

•    Understand the fundamentals of the CRISPR-Cas9 genome-editing tool.
•    Design a reliable CRISPR-based gene knockout experiment.
•    Functionally investigate a gene of interest in a non-model species.
•    Design and evaluate species-specific guide RNAs (gRNAs).
•    Efficiently synthesize gRNA and assemble optimized Cas9/gRNA mixes.
•    Screen for CRISPR-derived mutants using a number of genotyping methods.
•    Design genotyping strategies according to project needs.
•    Analyze Cas9-induced genomic variants by Illumina deep sequencing.
•    Critically interpret the results from a CRISPR-based KO experiment

 

For the full list of our courses and workshops, please visit: https://www.physalia-courses.org/courses-workshops/

 

 

Should you have any questions, please do not hesitate to contact us.

 

Best regards,

Carlo

 

 

 

 

 

 

 

 

--------------------

Carlo Pecoraro, Ph.D


Physalia-courses DIRECTOR

in...@physalia-courses.org

mobile: +49 17645230846