U6 promoter 'G' question

Stephen Forrow

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Apr 30, 2021, 5:52:07 AM4/30/21

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Quick question: If my guide sequence starts with a 'G', do I still need to include an additional 'G' at the end of the u6 promoter? For example, if cloning into pX330, do I still need to add the additional G to the end of the CACC over hang?

Many thanks,

Steve

--

Dr Stephen Forrow
Mouse Mutant Core Facility (PBA2B2)
Institut de Recerca Biomèdica - IRB
Parc Científic de Barcelona
C/ Baldiri Reixac 10-12,
08028 Barcelona
Spain

Tel 0034 93 403 9771
Fax 0034 93 4020260
stephen...@irbbarcelona.org

Ahmed Hassan

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Apr 30, 2021, 5:55:42 AM4/30/21

to Genome Engineering using CRISPR/Cas Systems

Dear Steve,

I hope you are doing well. With regards to your question, if your guide sequence starts with a G, you don't have to add an additional G at the start. Best of luck!

Kind regards,
Ahmed

stephen...@irbbarcelona.org

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May 2, 2021, 9:50:51 AM5/2/21

to Genome Engineering using CRISPR/Cas Systems

Thanks Ahmed!

best wishes,

Steve

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