CRISPR screens in primary T-cells.

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Ashwin Iyer

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Jul 28, 2025, 2:04:10 PMJul 28

to Genome Engineering using CRISPR/Cas Systems

Hi all,

Does anyone in the group have expertise in conducting CRISPR screens in primary T-cells? I have questions with respect to MOI, no. of cells to begin with for a genome wide library and the methodology for lentiviral transduction. I would really appreciate the help.

Best,

Ashwin Iyer

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