cell line-NG PAM- CRISPR/HDR
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Yatao Xiao
Oct 23, 2023, 11:10:34 PM10/23/23
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Hi, everyone!
I am in the process of constructing an EGFP knock-in cell line using the CRISPR-HDR system. Have any of you tried using CRISPR with an NG PAM coupled with a donor plasmid? Does it work as well as the NGG PAM?
Thank you very much!
Best regards,
Yatao
I am in the process of constructing an EGFP knock-in cell line using the CRISPR-HDR system. Have any of you tried using CRISPR with an NG PAM coupled with a donor plasmid? Does it work as well as the NGG PAM?
Thank you very much!
Best regards,
Yatao
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