Cas13 (C2C2) gRNA design tool

regulatingge...@gmail.com

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Jun 2, 2018, 9:53:57 AM6/2/18

to Genome Engineering using CRISPR/Cas Systems

Hello,

I am interested in targeting mRNA with Cas 13. Is there an online tool to design gRNA for Cas13?

Thanks.

Hadi Bayat

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Aug 13, 2018, 8:25:47 AM8/13/18

to Genome Engineering using CRISPR/Cas Systems

Hello,

you may find the following online tool useful:

http://bioinfolab.miamioh.edu/CRISPR-RT/interface/C2c2.php

Best,

Hadi

rutgerg...@gmail.com

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Mar 13, 2019, 5:14:40 AM3/13/19

to Genome Engineering using CRISPR/Cas Systems

Hi Hadi,

Thanks for the tool. Would it also allow design guides for Cas13d (by Konermann)

Best,

Rutger

Sudarshan Iyer

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May 28, 2019, 2:25:57 PM5/28/19

to Genome Engineering using CRISPR/Cas Systems

I would also like to know if anyone has experience designing guides for Cas13d. Have been trying to knockdown c-Myc with this system but have had no success with the first 3 gRNAs I have tried.

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