gDNA NGS PCR

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Jun 11, 2025, 11:09:04 AMJun 11

to Genome Engineering using CRISPR/Cas Systems

Hi All,

In your expert opinions, which combination of annealing temp and primer conc (nM) would you say is the optimum for PCR of gDNA for NGS sequencing?

Appreciate the help.

Best wishes,

Mehmet

Annealing Temps and Primer Concs.jpg

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