Analysis without replicates

[mat...@gmail.com]

Hi Jan,

I have a question.

Is it possible to do the analysis without replicates? My problem is that I only have two independent experiments and if I take them as replicates nothing is significant (except for the Z-ratio algorithm), which I guess is normal since it's expected a significant variation in sgRNA counts between completely different experiments.

So I'm trying to do it without replicates, but there is a message saying that the analysis cannot be performed with edgeR, but in fact any analysis with the other algorithms is performed at all...

Any feedback about this matter would be really appreciated.

Many thanks.

Matias