DESeq2 Hit calculation could not be performed.

[Shengb Suo]

Hi, thanks so much for this wonderful tool. When I uploaded original read counts files (two control samples and two treatment samples), it runs very well, but when I uploaded the normalized read count files (also these four files), it could not get results, and display "DESeq2 Hit calculation could not be performed." So do you have any suggestions? Does this tool do normalization for the uploaded original read counts?

Thank you so much for your kind help.

Best,

Shengbao

[Jan Winter]

Hi Shengbao,

sorry for having overseen your post!

Please only upload raw data or read count files that have not been normalized (the one CRISPRAnalyzeR will provide you are also not normalized -> just raw read counts).

If you provide normalized counts DESeq2 will throw an error, as it is used to normalize the reads by default.

best,

Jan