Genome Engineering using CRISPR/Cas Systems

Hyy On Tue, 26 Nov 2024, 9:03 pm Zena Shourbaji, <zenash...@gmail.com> wrote: Hello, I'

Are you passionate about hashtag#computational hashtag#biology? 🤔 Do you like to hashtag#work with

I am experiencing the same issue in Jurkat. It could be due to the inherent genes that you are trying

Hi, The result I got in LVP that I produced in 500000 cells in a 6-well with transfer:pspax2:vsvg in

Hi CRISPRians! This is Ahmed Elwakeel, a post-doc at the University of Cambridge. How Can I generate

Dear all, We are excited to announce that registrations are now open for the 2nd edition of our

Hi, everyone! Could you share some programs or softwares that can predict RNA:DNA hybrid structure

Hi good people, I was wondering if someone knows the exact reasons as to why the Calabrese library

Hello all, In my lab we are having a recurrent (but not permanent) problem when we try to transform

Hi Julia, We have now conducted a screen using the GeCKO V2 library and we recently came across your

Dear all, anybody having experience with the direct capture of proviral CRISPR sequences of screening

Hi all, I have been doing genome-scale positive selection screens with the Brie library and

Dear all, registrations are open for the 2nd edition of the online course "Introduction to

Hello all, Did anyone try transfection of pCMV-AN-HA vector with an insert of gene of interest in

These genes may be essential for cell survival, have you considered that? If that's the case, you

Id choose ROSA26 murine locus, as many publications validate it as a safe harbour locus for transgene

Hi everyone. The CRISPR plasmid pX 333 from Zhang lab has been optimised for cloning of 2 single

Bumping this to the top - curious if anyone has any input on a best practice for these libraries that

Hi Everyone, Where can I find the K562 cell line DNA sequence?

Hi all, I am trying to use CRISPOR to select target sequences for Crispr/Cas9 knock-in, but an error

1) whats the minimum coverage you would shoot for if you want a good number of hits. My understanding

Hello Meenu, I am facing a similar issue and was wondering whether you have meanwhile proceeded with

Hi, in this case you have no choice but to remove the stop codon of your gene then you can acquire

Hi, everyone! I am in the process of constructing an EGFP knock-in cell line using the CRISPR-HDR

Kindly suggest me any online tool used to design efficient sgRNA negative control Sequences. On Fri,

I'm actually in the process of using it right now. Our lab recently returned to a project that we

I'd say you should use a similar number of non-targeting guides as a control. But interested to

You need to change this line as well, 20 to 18. guide = read_sequence[start_index:(start_index + 20)]

Dear all, we have the last 4 seats available for the course "Introduction to CRISPR for ecology

Hello, fellow researchers, I'm working on a project cloning 11 guide RNAs into a Pxr003 plasmid