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Genome Engineering using CRISPR/Cas Systems

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Rhiannon Macrae, … Zeki Ilker Kanbagli3

1/13/20

Reminder: Registration is now open for our annual Genome Engineering Workshop!

Hi there Zhang lab! Are you going to arrange Genome Engineering Workshop this year too? Waiting for

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Reminder: Registration is now open for our annual Genome Engineering Workshop!

Hi there Zhang lab! Are you going to arrange Genome Engineering Workshop this year too? Waiting for

1/13/20

11/17/25

Need Partners for a legitimate (covert, discreet) transaction!

‎Need Partners for a legitimate (covert, discreet) transaction! ‎Clients wants to move assets ( ~

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Need Partners for a legitimate (covert, discreet) transaction!

‎Need Partners for a legitimate (covert, discreet) transaction! ‎Clients wants to move assets ( ~

11/17/25

11/17/25

Need Partners for a legitimate (covert, discreet) transaction!

‎Need Partners for a legitimate (covert, discreet) transaction! ‎Clients wants to move assets ( ~

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Need Partners for a legitimate (covert, discreet) transaction!

‎Need Partners for a legitimate (covert, discreet) transaction! ‎Clients wants to move assets ( ~

11/17/25

sambhavi senthil

10/29/25

Recommendations for generating Cas9 expressing cells

Hello everyone, We are planning to conduct an experiment using the LentiCas9-Blast lentiviral prep

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Recommendations for generating Cas9 expressing cells

Hello everyone, We are planning to conduct an experiment using the LentiCas9-Blast lentiviral prep

10/29/25

Camilla Bragonzi

10/27/25

Survival rate is too high in Jurkat transduced with lentivirus

Hi everyone, I am experiencing a very high survival rate when using Jurkat (suspension leukemia T

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Survival rate is too high in Jurkat transduced with lentivirus

Hi everyone, I am experiencing a very high survival rate when using Jurkat (suspension leukemia T

10/27/25

10/2/25

course on Introduction to CRISPR (13–16 Oct, online)!

Hi, There's still time to register for the Physalia course on Introduction to CRISPR for Ecology

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course on Introduction to CRISPR (13–16 Oct, online)!

Hi, There's still time to register for the Physalia course on Introduction to CRISPR for Ecology

10/2/25

8/14/25

Prime editing success?

Hello all, Has anyone had any success trying to use prime editing? I have tried a couple of times,

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Prime editing success?

Hello all, Has anyone had any success trying to use prime editing? I have tried a couple of times,

8/14/25

7/28/25

CRISPR screens in primary T-cells.

Hi all, Does anyone in the group have expertise in conducting CRISPR screens in primary T-cells? I

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CRISPR screens in primary T-cells.

Hi all, Does anyone in the group have expertise in conducting CRISPR screens in primary T-cells? I

7/28/25

7/22/25

Next generation CRISPRko genome-wide libraries from Vivlion GmbH

Hi CRISPR Community, My name is Jessica, and I work in functional genomics and CRISPR screening at

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Next generation CRISPRko genome-wide libraries from Vivlion GmbH

Hi CRISPR Community, My name is Jessica, and I work in functional genomics and CRISPR screening at

7/22/25

7/11/25

gDNA Amplification

Hi All, I have recently extracted genomic DNA from all of my samples and I have got WAY more genomic

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gDNA Amplification

Hi All, I have recently extracted genomic DNA from all of my samples and I have got WAY more genomic

7/11/25

7/7/25

Online Course – Introduction to CRISPR in October

Dear all, We are excited to announce the Introduction to CRISPR for Ecology and Evolution Studies

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Online Course – Introduction to CRISPR in October

Dear all, We are excited to announce the Introduction to CRISPR for Ecology and Evolution Studies

7/7/25

6/18/25

Plasmid request of NovaIscB-ωRNA

Hi everyone, I have to say that NovaIscB is the most exciting project for me recently, because I have

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Plasmid request of NovaIscB-ωRNA

Hi everyone, I have to say that NovaIscB is the most exciting project for me recently, because I have

6/18/25

6/11/25

Hi All, In your expert opinions, which combination of annealing temp and primer conc (nM) would you

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Hi All, In your expert opinions, which combination of annealing temp and primer conc (nM) would you

6/11/25

6/1/25

http://genome-engineering.org/ not accessible

Hi, I tried to access http://genome-engineering.org/ many times today and failed every time. Do you

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http://genome-engineering.org/ not accessible

Hi, I tried to access http://genome-engineering.org/ many times today and failed every time. Do you

6/1/25

Emre Deniz, Neelam Bodhale2

6/1/25

GeCKO website down

Hi I am facing the same issue. Thank you Neelam On Thursday, 12 July 2018 at 04:04:01 UTC-5 emr...@

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GeCKO website down

Hi I am facing the same issue. Thank you Neelam On Thursday, 12 July 2018 at 04:04:01 UTC-5 emr...@

6/1/25

Eduarda Baggio Paglia

5/29/25

Has Anyone Successfully Used pMBP-CRISPR-Cas12a? Looking for Advice

Hi all, My name is Eduarda, and I'm a PhD student in Chemical Engineering. I'm working on a

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Has Anyone Successfully Used pMBP-CRISPR-Cas12a? Looking for Advice

Hi all, My name is Eduarda, and I'm a PhD student in Chemical Engineering. I'm working on a

5/29/25

5/28/25

Seeking Recommendations for Inducible Cas9 Vectors and Nuclear Cas9 Antibodies

Dear all, I am working on generating inducible Cas9 (iCas9) iPSC lines for a while. We have a gRNA

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Seeking Recommendations for Inducible Cas9 Vectors and Nuclear Cas9 Antibodies

Dear all, I am working on generating inducible Cas9 (iCas9) iPSC lines for a while. We have a gRNA

5/28/25

5/21/25

Request for Guidance: No DNA Yield After CTAB Extraction from Fungal Sample

I hope this message finds you well. My name is Mazhar Ali, and I am currently working on fungal DNA

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Request for Guidance: No DNA Yield After CTAB Extraction from Fungal Sample

I hope this message finds you well. My name is Mazhar Ali, and I am currently working on fungal DNA

5/21/25

Mehmet Hazirci, Julia Joung5

5/21/25

gDNA PCR Optimisation

On Tuesday, May 6, 2025 at 6:13:38 PM UTC+1 juliaj...@gmail.com wrote: Hi Mehmet, Thank you for your

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gDNA PCR Optimisation

On Tuesday, May 6, 2025 at 6:13:38 PM UTC+1 juliaj...@gmail.com wrote: Hi Mehmet, Thank you for your

5/21/25

5/20/25

sgRNA amplification & NGS

Hi everyone, I am using the GeCKO v2 library and following the protocol from Joung et al. Nature 2017

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sgRNA amplification & NGS

Hi everyone, I am using the GeCKO v2 library and following the protocol from Joung et al. Nature 2017

5/20/25

Mehmet Hazirci, Julia Joung4

5/9/25

Technical Replicates and Assigning Rev Primers

Hi Mehmet, Technical replicates should have different reverse primers. After running PCR

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Technical Replicates and Assigning Rev Primers

Hi Mehmet, Technical replicates should have different reverse primers. After running PCR

5/9/25

5/9/25

gDNA Extraction from cell pellets

Hi All, I am attempting to extract gDNA from cell pellets using DNAzol, which is a guanidine

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gDNA Extraction from cell pellets

Hi All, I am attempting to extract gDNA from cell pellets using DNAzol, which is a guanidine

5/9/25

neelam bodhale2

5/7/25

Hi all, Here is another set of sequencing data of different batches of amplified GeCKO v2 mouse

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Hi all, Here is another set of sequencing data of different batches of amplified GeCKO v2 mouse

5/7/25

Yamimaka S, Narasimha Swamy Telugu2

5/3/25

Request for Lentiviral gRNA Vector Recommendations

Dear Yachten, To check in ipscs you can simply use synthetic sgRNAs. You can synthesise either in

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Request for Lentiviral gRNA Vector Recommendations

Dear Yachten, To check in ipscs you can simply use synthetic sgRNAs. You can synthesise either in

5/3/25

Faheem Kanwal, … lina shi6

5/1/25

Re: knock down large fragments using CRISPR/Cas9

HI Haruna, To prepare a 10 µL injection mix, use the following components based on a 1:2 ratio of

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Re: knock down large fragments using CRISPR/Cas9

HI Haruna, To prepare a 10 µL injection mix, use the following components based on a 1:2 ratio of

5/1/25

Jing Zhang, Xavier De Deken2

4/28/25

anyone has experience cleave human gDNA using crRNA and Cas12a?

Hi, To extract gDNA from cells (GeCKO human library) before PCR amplification of the integrated sgRNA

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anyone has experience cleave human gDNA using crRNA and Cas12a?

Hi, To extract gDNA from cells (GeCKO human library) before PCR amplification of the integrated sgRNA

4/28/25

Ahmed Elwakeel, Eric Shi2

4/8/25

sgRNA Library construction using LentiCRISPRv2 - Help!!!

maintaining a molar ratio of 3:1 (insert: vector) should be a good starting point. also make sure you

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sgRNA Library construction using LentiCRISPRv2 - Help!!!

maintaining a molar ratio of 3:1 (insert: vector) should be a good starting point. also make sure you

4/8/25

4/4/25

Help please with CRISPR by transient transfection of LentiGuide-Puro

Hello, I am attempting a slightly odd CRISPR protocol and wanted to run it by some experts please. I

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Help please with CRISPR by transient transfection of LentiGuide-Puro

Hello, I am attempting a slightly odd CRISPR protocol and wanted to run it by some experts please. I

4/4/25

4/1/25

Question on CRISPR screen analysis

Hello all, I have a FACS based CRISPR screen for which I can compare the sorted sample with presort.

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Question on CRISPR screen analysis

Hello all, I have a FACS based CRISPR screen for which I can compare the sorted sample with presort.

4/1/25

Mehmet Hazirci, Julia Joung2

3/30/25

No matches using count_spacers

Hi Mehmet, Thank you for your interest in our work. Based on your output, it looks like the key

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No matches using count_spacers

Hi Mehmet, Thank you for your interest in our work. Based on your output, it looks like the key

3/30/25

3/21/25

GeCKO library packaging

Dear all, Does anyone you ever tried packaging the GeCKO v2 library with pCMV-VSV-G plasmid instead

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GeCKO library packaging

Dear all, Does anyone you ever tried packaging the GeCKO v2 library with pCMV-VSV-G plasmid instead

3/21/25

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