Genome Engineering using CRISPR/Cas Systems

Hi there Zhang lab! Are you going to arrange Genome Engineering Workshop this year too? Waiting for

Hello all, Has anyone had any success trying to use prime editing? I have tried a couple of times,

Hi all, Does anyone in the group have expertise in conducting CRISPR screens in primary T-cells? I

Hi CRISPR Community, My name is Jessica, and I work in functional genomics and CRISPR screening at

Hi All, I have recently extracted genomic DNA from all of my samples and I have got WAY more genomic

Dear all, We are excited to announce the Introduction to CRISPR for Ecology and Evolution Studies

Hi everyone, I have to say that NovaIscB is the most exciting project for me recently, because I have

Hi All, In your expert opinions, which combination of annealing temp and primer conc (nM) would you

Hi, I tried to access http://genome-engineering.org/ many times today and failed every time. Do you

Hi I am facing the same issue. Thank you Neelam On Thursday, 12 July 2018 at 04:04:01 UTC-5 emr...@

Hi all, My name is Eduarda, and I'm a PhD student in Chemical Engineering. I'm working on a

Dear all, I am working on generating inducible Cas9 (iCas9) iPSC lines for a while. We have a gRNA

I hope this message finds you well. My name is Mazhar Ali, and I am currently working on fungal DNA

On Tuesday, May 6, 2025 at 6:13:38 PM UTC+1 juliaj...@gmail.com wrote: Hi Mehmet, Thank you for your

Hi everyone, I am using the GeCKO v2 library and following the protocol from Joung et al. Nature 2017

Hi Mehmet, Technical replicates should have different reverse primers. After running PCR

Hi All, I am attempting to extract gDNA from cell pellets using DNAzol, which is a guanidine

Hi all, Here is another set of sequencing data of different batches of amplified GeCKO v2 mouse

Dear Yachten, To check in ipscs you can simply use synthetic sgRNAs. You can synthesise either in

HI Haruna, To prepare a 10 µL injection mix, use the following components based on a 1:2 ratio of

Hi, To extract gDNA from cells (GeCKO human library) before PCR amplification of the integrated sgRNA

maintaining a molar ratio of 3:1 (insert: vector) should be a good starting point. also make sure you

Hello, I am attempting a slightly odd CRISPR protocol and wanted to run it by some experts please. I

Hello all, I have a FACS based CRISPR screen for which I can compare the sorted sample with presort.

Hi Mehmet, Thank you for your interest in our work. Based on your output, it looks like the key

Dear all, Does anyone you ever tried packaging the GeCKO v2 library with pCMV-VSV-G plasmid instead

Dear genome engineering members, My question is pertaining to PCR1 + PCR2 with regard to limited

Dear CRISPR community, I performed CRISPR Cas9 knockout of RIG-I and MDA5 in A549 cells. For control,

Hello All, I have been attempting prime editing following the premise in this paper. Search-and-

Dear all, First of all, I wish you all an Happy New year 2025 and all the best in your CRISPR Screen

Dear Colleagues, Figbox AI, a startup developed by researchers at the University of Oregon dedicated