Cel,
you can put (linear) boundaries for any of the parameters that you are
optimizing; these are inout as maximum and minimum values for each
parameter.
you can also add (nonlinear) boundaries for any variable, which also go
as maximum value and minimum value; these are added in the tab named
"constraints". In fact you can add maximum and/or minimum also of any
function of variables.
Be careful when adding boundaries for variables (and functions of
variables) as it is very easy to make the problem unsolvable. I would
add these one by one and only those strictly needed.
Pedro
--
Pedro Mendes, PhD
Professor and Director
Richard D. Berlin Center for Cell Analysis and Modeling
University of Connecticut School of Medicine
group website:
http://www.comp-sys-bio.org