Parameter estimation for different reaction rates

[cel.c...@gmail.com]

Hello,
I want to estimate two parameters and I want to do that with timecourse data which changes based on one reaction parameter.
So e.g. we want to estimate the values for parameter k1 and k2.
And I am interested in doing that w.r.t the concentration curve of AC_activity , which is an assignment type (not sure if that is important).
Anyways as a rule of thumb, the concentration curve of AC_activity, in particular the peaks for AC_activity look different with different k_releases.

I generated my dataset like this:

Time AC_activity k_release

0              0                1e-10
..              ...                1e-10
100          0                1e-10

################# (empty line)

0               0.5              1e-2

...              ...                 1e-2

100           0.5              1e-2

But somehow the evaluation runs fail. Can someone help with what I am doing wrong ?
Best Celvic

[Frank Bergmann]

Hello Celvic, 

I'm afraid we would need a little bit more information, especially how you mapped the data to your model. What are the dependent / independent model elements mapped to? If AC_activity is of type assignment, then they ought to be mapped as dependent element. K_release sounds like an independent value that you specify. 

I can offer that you send me the COPASI file + data and i can look over it to see why the evaluation fails. 

best

Frank

[cel.c...@gmail.com]

Hello Frank,

yes I chose AC_activity to be dependent and k_release to be independent and  I would appreciate your offer

Best Celvic