I'm currently working on a Systems biology project on Angiogenesis…
I wanted to extract Initial Concentration and Kinetic parameters I.e., Forward and Backward reaction kinetics of Molecular species.
In the Literature review, I came across papers which said I can get them by analyzing Western Blot images.
I followed these steps
1) ImageJ: To analyze Western Blot images and get plots of the band
2) Grabit function on MATLAB: Calibrate the plots and grab the point
3) Excel: Determine the concentration and find the initial and final rates
I’m getting stuck at finding the concentration as the points on the plot appear as a Line Chart and are difficult to analyze.
Can Somebody please help meet out with this?