This might be a very ridiculous general question but here we go:
I am trying to add a reaction for the metabolism of a drug by the enzyme CYP3A4. The problem is, all literature Vmax values are either in pmol/min/mg or pmol/min/pmol CYP3A4, while COPASI asks for Vmax units in the form: mol/h or mol/(h*L)
I am not an expert on enzyme kinetics, so I am not sure how one makes the conversion to fit the Vmax data into a COPASI model. Do we need the enzyme concentration (in g/L or in M), as well as the incubation volume?
Some insight would be highly appreciated.