Eppendorf Centrifuges

[Glendora Spink]

Thankyou Emily. This unit is obsolete and no longer supported by the manufacturer. I reached out to 2 of the 3 sponsors you suggested but they could not help me... hence the post looking for help on this forum.

Good morning. First off, have you tried calling Ozark? They are pretty knowledgeable on centrifuges. Secondly, error codes 9 thru 25 are electronics fault. Have you tried switching unit off and back on after a minute or so?

I spoke to someone at Ozark. They only carry the drive motor and gas spring for this model. I've tried switching on/off several different ways, waiting different periods of time. No joy. As I stated in my 2nd post, there is a cooked component on the main frequency converter board (relay). I'm looking for someone who may have experienced this and some advice on what to start tracing as the root cause. Eppendorf will not give me the time of day.

Thanks for the response. I pulled the cover and found that the Schrack RE030024 relay is cooked. There's a BBQ spot right behind it on the back side of the main frequency converter board along at least one trace between pins. Not sure if this is the main problem or just the result of a problem somewhere else in the circuit. Since this is a 3 layer board and I no longer have the electronic service manual (HD crash) I will be relegated to sending it out for repair.

If this unit is below serial number 12,000 it went obsolete over 10 years ago. Eppendorf doesn't carry or sell parts for it. Their Customer Service can only help as far as the Service manual troubleshooting allows. Eppendorf schematics are only block diagrams, no component level troubleshooting.

well I see you've already made your way to labwrench without any luck. error 22 is an electronics error and you've found the culprit, so next up is really just replacing that board, unless you want to accept liability and just replace the component. As far as tracking down the cause, unless it's a known error, I doubt you'll have any luck asking others.

It depends on what the component is and what it did. maybe there was too much imbalance in the unit, maybe it got a static charge or fell or was shaken during use, maybe it was a novice biomed, maybe just a design flaw hence the obsolete status.

So I sent the Main Frequency Converter Board out for repair and it should be coming back to me to install shortly. In the meantime, the person repairing the board recommended that I check the evaporator fan and the blower fan (under the bowl) as one or both were the culprits in taking out the board. I did that and found that the evaporator fan was fine but the blower fan has some issues and should probably be replaced. It's been a long time since I pulled one of these out of a 5810r and I no longer have my files on how to properly do this. There is no way for me to simply unbolt and slip it out to replace. The bowl insulation and tight quarters prevent this. I hoping that I don't have to unbolt the bowl and lift it out of the way to do this. Any suggestions or the ugly truth is appreciated. Many thanks. DISREGARD THIS POST. I WAS ABLE TO SUCESSFULLY PULL THE FAN.

Including quick-spin microcentrifuges, microcentrifuges, centrifuges, and refrigerated options for a majority of these, the Eppendorf line of centrifuges are versatile enough to fit into any laboratory. Pipette.com carries the complete line of Eppendorf Centrifuges, rotors, and accessories to meet all centrifugation needs. The Eppendorf name has become synonymous with creative design, innovative technology and reliable performance that stands the test of time. Improve your centrifugation work today with an Eppendorf Centrifuge!

The Eppendorf 5430 series of centrifuges offers a versatile 30 place centrifuge with an extensive choice of different rotors including microplates, 15mL and 50mL Falcon tubes and all common blood collection tubes. Available with the option of rotary controls or a keypad for ease of cleaning, the refrigerated version keeps the compressor at the back keeping the width narrow and access height low.

In 2023, the 5910Ri and 5427R Eppendorf centrifuges became the first centrifuges worldwide to receive the ACT label. The ACT label resembles a nutrition label, showing how products rate in various sustainability categories. The 5427R centrifuge now uses a hydrocarbon cooling liquid, propane, in place of the hydrofluorocarbons (HFCs) to reduce HFCs environmental impact. This blog covers the changes Eppendorf have made to two of their centrifuges to take the next step in becoming more environmentally friendly.

This overview given by ACT labelling provides an easy and intuitive way to evaluate the sustainability of the products. The products are rated and validated on the different Environmental Impact Factors (EIFs) on a scale from 1 to 10, 1 being the least environmental impact and 10 being the highest.

Eppendorf centrifuge 5910 Ri and 5427 R became the first centrifuges worldwide to receive the ACT label. Eppendorf have considered a variety of factors to reduce their carbon footprint including energy saving, longevity, ergonomics, and cooling liquids.

There are two main power-consuming steps when using a centrifuge: temperature management of the chamber and acceleration of the rotor. To reduce the power consumption, Eppendorf has introduced some new energy-saving features:

Eppendorf uses high-quality parts and a robust design to ensure years of performance and trouble-free use. They perform long-term stress tests on various functions of their centrifuges, such as lid opening and closing, in their R&D phase to ensure the materials they are using are long-lasting. Some of their centrifuges manufactured in the 60s are still running.

Refrigerated and cooled centrifuges will use a compressor and a cooling liquid/refrigerant to maintain the temperature required inside the centrifuge for the rotor and samples. The refrigerants commonly used are hydrofluorocarbons (HFCs) which are harmful to the environment, to help with this Eppendorf has reduced the amount of HFCs in their centrifuges when possible. Along with this they have developed a new 5427R centrifuge that uses a green cooling liquid - propane. This new model is an updated version of the previous 5427R centrifuge but features a new compressor and additional safety features to ensure smooth usage while using their new green hydrocarbon cooling propane.

Eppendorf supports My Green Labs mission to be accountable, consistent and transparent to work on reducing the company's environmental impact. Obtaining an ACT label for two of their centrifuges is the first step in Eppendorf's sustainability journey, as well as creating new Biobased consumables from a 2nd generation of renewable feedstock. More information on their Biobased tubes can be found here, and more information on their ACT sustainability label can be found here.

A laboratory centrifuge is a piece of laboratory equipment, driven by a motor, which spins liquid samples at high speed. There are various types of centrifuges, depending on the size and the sample capacity.[1]

Because of the heat generated by air friction (even in ultracentrifuges, where the rotor operates in a good vacuum), and the frequent necessity of maintaining samples at a given temperature, many types of laboratory centrifuges are refrigerated and temperature regulated.

Centrifuge tubes are precision-made, high-strength tubes of glass or plastic made to fit exactly in rotor cavities. They may vary in capacity from 50 mL down to much smaller capacities used in microcentrifuges used extensively in molecular biology laboratories. Microcentrifuges typically accommodate disposable plastic microcentrifuge tubes with capacities from 250 μL to 2.0 mL.

Glass centrifuge tubes can be used with most solvents, but tend to be more expensive. They can be cleaned like other laboratory glassware, and can be sterilized by autoclaving. Small scratches from careless handling can cause failure under the strong forces imposed during a run. Glass tubes are inserted into soft rubber sleeves to cushion them during runs. Plastic centrifuge tubes, especially tend to be less expensive and, with care, can be just as durable as glass. Water is preferred when plastic centrifuge tubes are used. They are more difficult to clean thoroughly, and are usually inexpensive enough to be considered disposable.

Disposable plastic "microlitre tubes" of 0.5ml to 2ml are commonly used in microcentrifuges. They are molded from a flexible transparent plastic similar to polythene, are semi-conical in shape, with integral, hinged sealing caps.

Larger samples are spun using centrifuge bottles, which range in capacity from 250 to 1000 millilitres. Although some are made of heavy glass, centrifuge bottles are usually made of shatterproof plastics such as polypropylene or polycarbonate. Sealing closures may be used for added leak-proof assurance.

The load in a laboratory centrifuge must be carefully balanced. This is achieved by using a combination of samples and balance tubes which all have the same weight or by using various balancing patterns without balance tubes.[2] It is an interesting mathematical problem to solve the balance pattern given n slots and k tubes with the same weight. It is known that the solution exists if and only if both k and n-k can be expressed as a sum of prime factors of n.[3] Small differences in mass of the load can result in a large force imbalance when the rotor is at high speed. This force imbalance strains the spindle and may result in damage to the centrifuge or personal injury. Some centrifuges have an automatic rotor imbalance detection feature that immediately discontinues the run when an imbalance is detected.

Before starting a centrifuge, an accurate check of the rotor and lid locking mechanisms is mandatory. A spinning rotor can cause serious injury if touched. Modern centrifuges generally have features that prevent accidental contact with a moving rotor as the main lid is locked during the run.

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