Centrifuge 5415r

[Samantha Figueredo]

Centrifuge5415 R has the same user friendliness and all the tried and tested technical features of the non-refrigerated 5415 D, including the almost indestructible maintenance-free drive, which requires only 13 seconds to accelerate to the maximum speed of 13,200 rpm (max. rcf: 16,000 x g).

The rotor runs so smoothly that particles that have already been pelleted are not unintentionally resuspended. Centrifuge 5415 R enables rapid and reliable refrigerated centrifugation in a temperature range between 0 C and 40 C. Temperature-sensitive samples are maintained safely at 4 C, even at maximum speed. In the stand-by mode, the centrifuge then can be set to the desired temperature. With the aid of the Fast Cool function, refreshing 4 C can be attained within a mere 16 minutes. One undisputed innovation on Centrifuge 5415 R is the new motorized lid latch. Two built-in gripper arms close

the newly designed lid automatically.

Hi This error is failing main PCB board. it requires testing and repair. Unfortunitly, i can not point you toward one component. everytime it is different component in the board. We fix and repair 5415R centrifuges. I fixed many over the past years for many companies and universities.

Our 5415R centrifuge displays Error 20 and Error 21. Contacting the eppi tech rep says Error 20 refers to an issue with the temperature sensor, while Error 21 refers to an issue with the condensor sensor.

Hi Oski Bear.... Error 20 is the temp sensor in the rotor chamber. You can use the Service Program 10 to test these sensors. Turn the unit off, while pushing down on the RPM control knob, turn the unit on to enter the service mode.

I've sent a number of Eppendorf boards to Sahara Simba Electronic for repair. They do component level repair on Eppendorf and other products. They have allowed me to do a lot more repairs on Eppendorf products.

Hello, we were finally able to find the centrifuge problem. Error 6 was due to a bad contact between the controller board and the power board. The pressure screws were somewhat loose. We adjusted them and the error disappeared.

Our centrifuge is now giving this error 6. After taking it apart I was able to locate the brake resistor. It has 3 pins, 2 on the ends and one closer to the middle of the resistor. One of the pins looks like it can be bridged to the middle one but has come desoldered. My first thought is that this is used for testing purposes but the comment by Filipe above suggests it should be attached during normal function?

Refrigerated tabletop centrifuges are quite expensive. Although discontinued, a refurbished Eppendorf 5415R (the model we use) still sells for $1-2K. The second of our two Eppendorf 5415R broke already more than a year ago and since then, we have been either centrifuging in the cold room or using the neighboring lab's cold fudge. But having two broken centrifuges of the same type, but different failure symptoms begged to assemble one functioning out of the remains.

One of the centrifuge rotors was stuck (could not be turned even by hand) and the other fuge was giving sparks and smoke when starting a run. I had postponed this repair since I thought it might take much time. However, when I opened the first centrifuge, I immediately could identify the part that I needed to graft from the other fuge. The whole repair lasted less than one hour. There was a blown resistor next to the cooling fan, which was easily replaced with the part from the second fuge. You can see the broken brass-colored resistor (CGS HSA50 resistor, available from Amazon for $13) in the front of the picture left to the rotor.

The failure of both centrifuges was apparently caused by liquid getting into the inner workings of the fuge. The rotor of the second centrifuge could not turn anymore because it was corroded from the salt and water. Also, the resistor had apparently been soaked (it is located immediately below the cooling grill on the top back of the fuge).

The centrifuge works again including the cooling. I do not know for how long we will enjoy it, because it was already 2nd hand when we bought it in 2014. But even if it lasts only for another year, the repair was worth it.

This type of repair might become easier in the future because if I am not mistaken, the EU is planning to mandate that manufacturers make service and repair manuals available to third party repair shops (this mandate exists already for car manufacturers to ensure that independent car repair shops can survivce).

For non-chiller units, remove the four side screws, and the entire housing should come right off. If not, turn it upside down and note the rusty crust that's gluing the metal frame to the plastic! Slide a knife, or pry to break the bond. Then invert again, lift the housing carefully and reach inside to remove the ribbon cable from the PCB (pinch the plastic connector sides to release.) There are also two ground cables and the release-string to remove. (Also note, if you want to remove the main PCB, must unscrew the four screws on the BOTTOM of the centrifuge, not the four screws on the PCB itself.

I've now seen more than one Eppendorf lab centrifuge with the same problem: sudden death, as if a fuse has blown, but the fuses are fine.The LCD remains blank. Simple cure: increase a 2.2uF capacitor value to 10uF or higher (but stay below 100uF.) This fixes an apparent problem of slow-aging of the "boot-circuit" in the main switching supply.

DETAILSIn both dead units the cause was the same: switching supply failure when the "bootstrap" startup circuit can't run the supply, but goes into constant resetting. If the supply could just start itself up once, everything else works fine. In the past with other systems, this failure comes from dead dried-out electrolytic capacitors. But replacing the large 2.2uF and 220uF caps in the centrifuge didn't help, and the values of the original components were OK.

Next I look at the circuitry around the UC2844 (which is live, lethal. Floating-at-170VDC-plus-120v). I use a (electrically floating, dangerous!) scope to monitor the 16-volt input to the Vcc pin of the UC2844 controller chip. (This Vcc is also found at the input pin of that other, non-isolated LM7805 regulator in the center of the board. The regulator tab is also the chip's common.) A 2nd scope channel monitors the voltage generated by the high-freq transformer, found at the cathode of a SMT diode next to the 220uF cap. During each restart, the 2.2uF cap must rise to 16V, then the chip turns on, and the little VOGT 50KHz transformer should take over, charging up the adjacent, larger 220uF cap, and power everything. On the scope, the 2.2uF does rise to 16V, turning on the UC2844. That voltage now decreases rapidly, since the 2.2uF is acting as the entire power supply. The 50KHz switcher runs for a moment, and it's output voltage (across the 220uF) rises rapidly. But before it has time to rise to 10V, the voltage on the 2.2uF has fallen below 10V, and the UC2844 shuts down. Then repeat the whole sequence. But, it just missed normal startup byabout half a volt! One cap voltage falls, the other rises, and they must pass each other in order to turn on the supply. There's not enough millijoules in the 2.2uF to last for the whole millisecs needed to boot up the switching supply.

So, maybe there's a shorted turn in that VOGT high-freq transformer block. Or maybe the LCD front panel is now drawing a few mA extra during the rising of the isolated 5V supply. Or maybe it was always supposed to be a 22uF capacitor, not 2.2uF? The voltages and RC periods are very close to the edge of failure, and any little change can push it over the threshold. Could I try replacing the little brown VOGT high-freq transformer? Nope, not available. So, kludge it: swap the 2.2uF capacitor with 10uF 25V tantalum. Yep, that did it. Starts up now just fine. (Hmm, in hindsight I probably should have used a 22uF or 47uF rather than a 10uF, just to keep the same failure from happening again in a couple of years!) ANOTHER TRICK: stand up the capacitor on 1cm leads, then to keep it cool, bend it away from the hot TO-220.

With their space-saving design and user-friendly operating concept, these centrifuges are ideal for everyday routine tasks.

24 Eppendorf micro test tubes can be centrifuged simultaneously in a 45 fixed-angle rotor at 16,100 rcf (13,200 rpm).

The basic functions can be selected using the "SHORT" key and the "TIME" and "SPEED" dial.

The values appear in the display in digital form.

The Centrifuge 5415 D is a non-refrigerated bench-top centrifuge and the Centrifuge 5415 R is a refrigerated bench-top centrifuge. Both are used in research and routine laboratories in the biosciences, industry, clinics and chemistry.

The Eppendorf 5415 series centrifuges have been discontinued by the manufacturer and replaced by the 5424 and 5424 R models. However, a number of units are always available for sale on LabX. Rotors and accessories for the 5415 D/R are still available for sale from Eppendorf, or there is a wide selection of rotors and parts available on LabX as well.

Find online auctions and classified ads for Eppendorf products and more on the LabX Marketplace. Browse ads and contact the seller directly or request a quote for more details. Buy and Sell Eppendorf Equipment and Accessories today on LabX!

This Eppendorf 5415R (5426) Refrigerated Microcentrifuge with Rotor with F-45-24-11 rotor is in excellent condition and has been fully refurbished and tested by out scientific and technical staff and comes with a 90-day warranty.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

3a8082e126