Dynamic FBA (Optimization of lipid production with a genome-scale model of Yarrowia lipolytica)

[Gaurav Shahare]

I am working on  Y. lipolytica reconstruction.

Here i am trying to maximize the lipid production by nitrogen starvation

 

I am changing the objective function to 

model = changeObjective (model, 'LDparticle_SC_e_tp'); %lipid production

on running the dfba i am getting the biomassVec as output.

 

I want lipid concentration instead of biomass concentration at each time step by using dfba? Please help

I also attaching the matlab code which came along with paper.

substrateRxns = {'EX_glc_e_','EX_cit_e_'};

initConcentrations = [20,0.085] ;  %20 g/l of glucose and 85mg/l nitrogen

initBiomass = 0.03;

timeStep = 0.5;

nSteps = 40;

plotRxns = {'EX_glc_e_',};

[concentrationMatrix,excRxnNames,timeVec,biomassVec] =  dynamicFBA(model,substrateRxns,initConcentrations,initBiomass,timeStep,nSteps,plotRxns);