Help with compounds production

[dtusso]

Hello everyone

I am evaluating ethanol and xylitol production in a yeast model. Production of these two metabolites occurs under anaerobic conditions. When I change the conditions to anaerobic with biomass maximization as the objective function, the model forms biomass but is not produce the metabolites of interest. I tried placing the maximization of the exchange reactions of ethanol and xylitol as an objective function, and it forms the compounds, verifying that there are no gaps in the metabolic pathways.

On the other hand, going back to the biomass equation as an objective function, other unwanted compounds are formed, so I block the exchange reactions of those metabolites. I get ethanol but not xylitol, and the growth rate decreases.

Can anyone help me with any suggestions? I would appreciate it.

Thank you

[Ronan M.T. Fleming]

https://opencobra.github.io/cobratoolbox/stable/tutorials/tutorialOptForce.html

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Mr. Ronan MT Fleming B.V.M.S. Dip. Math. Ph.D.
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Associate Professor,
School of Medicine,
National University of Ireland, Galway.
&
Assistant Professor,
Division of Systems Biomedicine and Pharmacology,
Leiden Academic Centre for Drug Research,
Faculty of Science,
Leiden University.

https://www.universiteitleiden.nl/en/staffmembers/ronan-fleming

&

Coordinator of the Horizon Europe project "Reconstruction and Computational Modelling for Inherited Metabolic Diseases" (Recon4IMD)

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Peer-reviewed publications: https://goo.gl/FZPG23
Mobile:  +353 852 109 806
Skype: ronan.fleming
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