I am trying to predict the blood levels of glucose (and a few other metabolites) using the whole-body model under a given diet. How do you recommend to do this?
On a related note, I wonder if glucose in the blood compartment, "glc_D[bc]", is under steady-state mass balance. When I sum the flux of all reactions in which "glc_D[bc]" appears as a reactant or product, times it's stoichiometric coeff. I get a value in the order of -3.05E-05, which is not close enough to zero implying that maybe the steady-state mass balance is not imposed for it (simulations under EU average diet). This happens for other metabolites I tested too.
Thank you in advance.
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Thanks for this feedback. It sounded like I had some solver issues, which I was able to resolve, and now I am able to get the same solutions as yours. Nevertheless, since all metabolites in the blood compartment are under the steady-state mass balance, I still wonder if there is any way of predicting the serum levels of glucose and other metabolites using the WBM (without performing dynamic simulations).
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Thanks for the quick response! Yes, I was aware of this paper and will look into it further, but I referring to predicting serum levels of metabolites using steady-state simulations (that’s what I meant by “without doing dynamic simulations.” Does your response mean that we cannot do this using steady-state simulations and have to use dynamic FBA?