introducing new media composition in the fba

[Annie M]

I would like help on how to introduce a new media into the fba to test growth.

Also I would like help on how to run a comparison between my model predictions and experimental data.

Best,

Anny

[Bruno Batista]

  Hello Maisa,

you can check the medium using "model.medium".

If you are running it in python, you will see a dic with exchange reactions of metabolites like :

"{ EX_XX: 1000 , EX_compound: 1000}"

The medium is a constraints and you can insert another dict to your model as below:

medium_1 = {EX_metabolite_1: 1000 ,   EX_metabolite_ 2  : 1000}

medium_2 = {EX_metabolite_X: 1000 ,   EX_metabolite_ Y  : 1000}

model.medium = medium_1, run FBA and

model.medium = medium_2, run FBA again.

I think it will help you

best regards,

Bruno