Anaerobic condition using Yeast 7.6 - no growth

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Lucas Carvalho

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Jun 1, 2022, 9:47:27 AM6/1/22

to cobra pie

Hello everybody.

Has anyone who uses cobrapy ever achieved the yeast grow in anaerobiosis (blocking oxygen reaction) using the Yeast 7.6 model?

Even changing the sterol reactions boundaries (as suggested in Yeast 6, and it works for the iMM904) I can't get any growth.

I would like to know which boundaries I should change from which reactions.

Sterol reactions:

episterol ("r_1753")
ergosterol ("r_1757")
fecosterol ("r_1788")
lanosterol ("r_1915")
zymosterol ("r_2106")
ergosta-5,7,22,24(28)-tetraen-3beta-ol ("r_2137")