Normalization of CLIP peaks by RNA abundance

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Anil Kesarwani

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Mar 3, 2016, 4:08:53 PM3/3/16
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Hi,
 
I am using CLIPer for iCLIP data analysis. I was wondering how to normalize peaks by transcript  abundance (calculated as FPKM from RNA-seq). I read in several articles that normalization with transcript level could help remove spurious peaks. I therefore performed RNA-seq as well, together with CLIP-Seq for the corresponding sample. Could please help me out. Your any response is highly appreciated.

Thanks.

Best.
Anil
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