I have Downloaded Databases Bacteria Virus separately and Fungi separately. Is this will create problem ?

[kathirvel k]

Hi Clark Users 

I have Downloaded Bacteria Virus Databases separately and Fungi separately, by following command ;

./set_targets.sh Dir_DB/ bacteria viruses --species

./set_targets.sh DBT/ fungi --species

Then I am performing  ./classify_metagenome.sh -O /home/DIR_DB/Bacteria/GCF_000005825.2_ASM582v2_genomic.fna -n 32 -R result

The K-mer step will take both bacteria viruses and fungi databases correctly or not ? 

Is this will create problem while ./buildSpacedDB.sh ? or am i missing anything? Thanks in Advance.

[Rachid]

Hi Kathirvel,

When you call "./classify_metagenome.sh" it will look up the latest database settings, so it is what the last call of "set_targets" instructed.

So in your example you've shown below, the database is defined for "fungi" at the species level.

Best,

Rachid

[Rachid]

Hi Kathirvel,

I am checking-in to see whether my answer addressed your question. Does it make sense?

Thank you for your help!