Couldn't create circos plot

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Soumya Ramankutty

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Dec 23, 2022, 2:38:22 PM12/23/22
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Hi, 
    I am trying to create a circos plot using the following data: 

chr    -    assembly_contig_1    ch01    1    12773    lum70chr_11
chr    -    assembly_contig_2    ch02    107    12722    lum70chr_12
chr    -    assembly_contig_3    ch03    80    7881    lum70chr_14
chr    -    assembly_contig_4    ch04    2    5805    lum70chr_13
chr    -    assembly_contig_5    ch05    2    5684    pred_vvd
chr    -    assembly_contig_6    ch06    500    4740    purple_vvd
chr    -    assembly_contig_7    ch07    432    3970    chr_1
chr    -    assembly_contig_8    ch08    785    4379    pyellow_vvd
chr    -    assembly_contig_9    ch09    1    3542    red_vvd
chr    -    assembly_contig_10    ch10    994    3171    lum70chr_16
chr    -    assembly_contig_11    ch11    1    695    lum70chr_18
chr    -    assembly_contig_12    ch12    223    2835    lum70chr_22
chr    -    assembly_contig_13    ch13    1    2598    lum70chr_3
chr    -    assembly_contig_14    ch14    134    2773    lum70chr_6
chr    -    assembly_contig_15    ch15    134    2773    chr_un
chr    -    assembly_contig_16    ch16    473    2721    ppurple_vvd
chr    -    assembly_contig_17    ch17    389    2472    lum70chr_9
chr    -    assembly_contig_18    ch18    1011    1874    lum70chr_17
chr    -    assembly_contig_19    ch19    2    1689    lum70chr_20
chr    -    assembly_contig_20    ch20    2    1689    blue_vvd


My configure file is as follows:

karyotype = simple_data.txt

<ideogram>

<spacing>
default = 0.005r
</spacing>

radius              = 0.80r
thickness           = 20p
fill                = yes
stroke_color        = dgrey
stroke_thickness    = 2p

#BANDS
#show_bands          = yes
#fill_bands          = yes
#band_transparency   = 4

#LABELS
show_label          = yes
lable_font          = default
lable_radius        = 1r + 75p
lable_size          = 30
lable_parallel      = yes

</ideogram>

<image>
#Included from Circos distribution.
<<include etc/image.conf>>
#To modify the size of the ouput image , default is 1500
#radius*            = 3000p
</image>
<<include /etc/colors_fonts_patterns.conf>>
<<include /etc/housekeeping.conf>>


I am not getting an image but getting an error as below : 

debuggroup summary 0.17s welcome to circos v0.69-8 15 Jun 2019 on Perl 5.034000
debuggroup summary 0.17s current working directory /home/sowmiya/Desktop/Data/Phage/Circos
debuggroup summary 0.17s command /home/sowmiya/Tools/circos-0.69-9/bin/circos -conf circos.conf
debuggroup summary 0.17s loading configuration from file circos.conf
debuggroup summary 0.17s found conf file circos.conf
debuggroup summary 0.32s debug will appear for these features: output,summary
debuggroup summary 0.32s bitmap output image ./circos.png
debuggroup summary 0.32s SVG output image ./circos.svg
debuggroup summary 0.32s parsing karyotype and organizing ideograms
debuggroup summary 0.32s karyotype has 20 chromosomes of total size 81,532
debuggroup summary 0.33s applying global and local scaling
debuggroup summary 0.33s allocating image, colors and brushes
debuggroup summary 2.11s drawing 20 ideograms of total size 81,532
debuggroup summary 2.11s drawing highlights and ideograms
Use of uninitialized value in subroutine entry at ../lib/Circos/Font.pm line 164.
Use of uninitialized value $radius in division (/) at ../lib/Circos/Text.pm line 317.
Illegal division by zero at /../lib/Circos/Text.pm line 317.


Hope someone would help me to rectify this error. 

Thank you. 

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