Bipartition file missing from RAxML-HPC BlackBox analysis

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Dilara Jayasekera

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Dec 29, 2024, 10:30:57 AM12/29/24
to CIPRES Science Gateway Users
Hello!

I conducted a phylogenetic analysis of 360 ITS sequences using the RAxML-HPC BlackBox (8.2.12) tool on CIPRES. However, when I run the task, the bipartition file is not available under the output. I have attached screenshots of the parameters that I specified along with task message. 

Thank you
Best regards
Dilara
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Pfeiffer, Wayne

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Dec 29, 2024, 2:18:29 PM12/29/24
to Dilara Jayasekera, CIPRES Science Gateway Users, Pfeiffer, Wayne
Hi Dilara,

Your job did not run successfully because some of the taxon labels in your fasta file contain the character “)”. That is not allowed by RAxML.

* Please remove those labels and resubmit your job.

Best regards,
Wayne

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Dilara Jayasekera

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Dec 29, 2024, 10:25:17 PM12/29/24
to Pfeiffer, Wayne, CIPRES Science Gateway Users

Hi Wayne,

Thank you for the suggestion. I redid the phylogenetic analysis by removing '(', however, I'm still not getting the bipartition file. Perhaps, a change in the paraneters is required? I have attached a screenshot of the recent task message. 

Thank you
Best regards
Dilara


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Screenshot (1081).png
Task message.png

Pfeiffer, Wayne

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Dec 29, 2024, 10:42:34 PM12/29/24
to Dilara Jayasekera, CIPRES Science Gateway Users, Pfeiffer, Wayne
Hi Dilara,

The first error message in the stdout.txt file is

ERROR: Taxon Name "GU300095.1_Xylaria_hypoxylon_isolate_95082001_18S_ribosomal_RNA_gene_partial_sequence_internal_transcribed_spacer_1_5.8S_ribosomal_RNA_gene_and_internal_transcribed_spacer_2_complete_sequence_and_28S)" is invalid at position 199, it contains illegal character )
Illegal characters in taxon-names are: tabulators, carriage returns, spaces, ":", ",", ")", "(", ";", "]", "[", "’" 

Note that the illegal character in your fasta file is a right parenthesis: “)”. That is the character that you need to remove.

Best regards,
Wayne

Screenshot (1080).pngScreenshot (1081).pngTask message.png

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