RAXML bootstrapping did not complete the 1,000 replicates as being setup

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Jeremiah Sia

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Mar 26, 2025, 9:49:13 PMMar 26
to CIPRES Science Gateway Users
Dear sir/mdm., 

I would like to inquire why the bootstrapping of the RaxML-NG program i was running did not complete the 1,000 replicates as i have set up. It converged at around 250 replicates. Please advice on how to increase the replicates number before convergence. 

Thank you.

Jeremiah

Pfeiffer, Wayne

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Mar 27, 2025, 5:17:26 AMMar 27
to Jeremiah Sia, CIPRES Science Gateway Users, CIPRES ADMIN
Hi Jeremiah,

* Please send your _JOBINFO.txt file to cipre...@sdsc.edu.

Thanks,
Wayne

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Miller, Mark

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Mar 27, 2025, 1:28:47 PMMar 27
to Pfeiffer, Wayne, Jeremiah Sia, CIPRES Science Gateway Users, CIPRES ADMIN

Hi Jeremiah,

The run stopped because the run reached convergence.
If you aren’t happy with the result, you can up the convergence stringency.

Clone the job, find the parameter: Specify the bootstrap cutoff value

And make it more stringent.

 

Hope that helps. Let me know if it doesn’t.

 

Mark

Jeremiah Sia

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Mar 28, 2025, 3:44:46 AMMar 28
to Miller, Mark, Pfeiffer, Wayne, CIPRES Science Gateway Users, CIPRES ADMIN
Dear Mark and Wayne,

Thank you so much for your replies! It worked after adjusting the bootstrap cutoff value.

Thanks again. 

Regards,
Jeremiah
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