Graphpad Prism Extension

[Onfroi Baird]

Prism10 introduced a new file format that uses the ".prism" file extension. This new file format is the default for Prism 10 (and will likely be used with future versions of Prism as well). Because Prism 9 (and all other earlier versions) were released before this file format was created, these earlier versions of Prism cannot open files with the .prism file extension. If you have an older version of Prism on your computer and you try to open a file with the .prism extension, you will see one of the error messages detailed below.

The easiest way to resolve these issues is to upgrade to Prism 10. You'll be able to open files using the .prism file extension, and you'll still be able to open and work with files in older formats in Prism 10 (Compatibility Mode was introduced to allow you to keep working with files using the .pzfx and .pzf file extensions).

In case you need to share a file with a colleague who is using an older version of Prism, Prism 10 also allows you to save your files inot one of the older file formats (using either the .pzfx or .pzf extensions). Simply use the File > Save As... command.

Don't be confused by the "data format" phrasing here. This is just a result of the way the new file format was created and organized. It's still a Prism file, and will open in Prism 10 just as you'd expect.

this is the interactive view

but still I cant download the file or interpretive the results

edit: I am not sure though if this is visualization of my data or saved on the website. i just choose interactive tab after dragging my qzv

On the first page, you can scroll ALL the way down to download the CSV that tells you how many sequences per sample (which is important). You can plot them using another program such as graphpad (prism).

thanks, I am trying. how to know where to trim? what is the accepted threshold? also I have paired-end so how to make sure they overlap? Finally, how long may it take to run as I read it may take 4 days? I only trying on 6 samples not all my samples.

so, Ben which numbers should we choose for trim?

based upon what?

any tutorial to help me understand and decide?

thanks

edit: because I keep on getting same error (1/2?) no such option: --p-trim-left (Possible options: --p-trim-left-f,

Trim #s should be based on if you have extra NT that you think may have been left from your primers. Here your trim # can be zero - I sometimes out of habit will trim 8-13 off of the forward and reverse. This should not impact your DADA2 results. Ben

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