convert bed to wig file

[swa...@gmail.com]

Dear User,

I am new in QuEST. I have chip-seq data and already done the peak calling, after the peak calling, I proceed to get unique peaks by bedtools. Now I have the bed file, but I would like to convert it to wig file, which can show the magnitude in the genome browser. Is this possible to do that using QuEST and how?

Thanks a lot for the help. Any input would be appreciated.

Emma

[Anton]

Hi Emma,

yes, it is possible. Check out the QuEST tutorial.

QuEST provides normalized wig score files that can be viewed using the genome browser. They should be in the tracks/wig_profiles directory of your QuEST analysis.

Best, Anton